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首页> 外文期刊>BMC Neuroscience >Previous infection with Staphylococcus aureus strains attenuated experimental encephalomyelitis
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Previous infection with Staphylococcus aureus strains attenuated experimental encephalomyelitis

机译：先前感染金黄色葡萄球菌菌株可减轻实验性脑脊髓炎

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Background Bacterial superantigens are potent T cell activators that can activate T cells with specificity for antigens of the central nervous system (CNS). In this study, we compared the effect of two S. aureus strains on experimental autoimmune encephalomyelitis (EAE) development. C57BL/6 female mice were infected with S. aureus ATCC 51650, which produces toxic shock syndrome toxin 1 (TSST-1+) or S. aureus ATCC 43300, which does not produce toxins (TOX-). Three days later, the animals were subjected to EAE induction by immunization with myelin oligodendrocyte glycoprotein (MOG). The weight variation, disease incidence and clinical score were recorded daily. Cytokines and Foxp3+ regulatory T cells in the brain were evaluated during the acute disease phase. Cytokines and Foxp3+ regulatory T cells in the spleen and histopathological analysis of the CNS were assessed during the chronic stage. Results Previous infection with both strains similarly decreased the clinical score; however, only the TSST-1+ strain clearly diminished inflammation in the CNS. The infections also modulated cytokine production in the spleen and CNS. Reduced production of IL-5 and IL-10 was detected in MOG-stimulated spleen cultures in the TOX- and TSST-1+ infected groups, respectively. In S. aureus stimulated cultures, there was an increased production of IFN-γ and IL-10 in both infected groups and an increased level of IL-5 in the TSST-1+ group. CNS infiltrating cell cultures from previously infected mice produced less IL-17 in response to MOG and more IFN-γ in response to S. aureus stimulation. Conclusions These results indicated that both strains attenuated clinical EAE manifestations, but only TSST-1 clearly decreased CNS inflammation.
机译：背景技术细菌超抗原是有效的T细胞激活剂，可以激活对中枢神经系统（CNS）抗原具有特异性的T细胞。在这项研究中，我们比较了两种金黄色葡萄球菌菌株对实验性自身免疫性脑脊髓炎（EAE）发育的影响。 C57BL / 6雌性小鼠感染了产生毒性休克综合症毒素1（TSST-1 +）的金黄色葡萄球菌ATCC 51650或不产生毒素（TOX-）的金黄色葡萄球菌ATCC 43300。三天后，通过用髓磷脂少突胶质细胞糖蛋白（MOG）免疫对动物进行EAE诱导。每天记录体重变化，疾病发生率和临床评分。在急性疾病阶段评估了大脑中的细胞因子和Foxp3 +调节性T细胞。在慢性阶段评估脾脏中的细胞因子和Foxp3 +调节性T细胞，以及中枢神经系统的组织病理学分析。结果两种菌株先前的感染均类似地降低了临床评分。但是，只有TSST-1 +菌株可以明显减轻中枢神经系统的炎症。感染还调节了脾脏和中枢神经系统中细胞因子的产生。在被TOX-和TSST-1 +感染的组中，在MOG刺激的脾脏培养物中分别检测到IL-5和IL-10的产生减少。在金黄色葡萄球菌刺激的培养物中，两个感染组的IFN-γ和IL-10产量增加，TSST-1 +组的IL-5水平升高。来自先前感染小鼠的CNS浸润细胞培养物响应MOG产生的IL-17减少，响应金黄色葡萄球菌刺激的IFN-γ产生更多。结论这些结果表明，两种菌株均减弱了临床EAE表现，但只有TSST-1明显降低了CNS炎症。

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