...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Neuroscience >Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction
【24h】

Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

机译：对生理和病理条件进行建模以研究脑功能和功能障碍中的周细胞生物学

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Brain pericytes ensheathe the endothelium and contribute to formation and maintenance of the blood–brain-barrier. Additionally, pericytes are involved in several aspects of the CNS immune response including scarring, adhesion molecule expression, chemokine secretion, and phagocytosis. In vitro cultures are routinely used to investigate these functions of brain pericytes, however, these are highly plastic cells and can display differing phenotypes and functional responses depending on their culture conditions. Here we sought to investigate how two commonly used culture media, high serum containing DMEM/F12 and low serum containing Pericyte Medium (ScienCell), altered the phenotype of human brain pericytes and neuroinflammatory responses. Pericytes were isolated from adult human brain biopsy tissue and cultured in DMEM/F12 (D-pericytes) or Pericyte Medium (P-pericytes). Immunocytochemistry, qRT-PCR, and EdU incorporation were used to determine how this altered their basal phenotype, including the expression of pericyte markers, proliferation, and cell morphology. To determine whether culture media altered the inflammatory response in human brain pericytes, immunocytochemistry, qRT-PCR, cytometric bead arrays, and flow cytometry were used to investigate transcription factor induction, chemokine secretion, adhesion molecule expression, migration, phagocytosis, and response to inflammatory-related growth factors. P-pericytes displayed elevated proliferation and a distinct bipolar morphology compared to D-pericytes. Additionally, P-pericytes displayed lower expression of pericyte-associated markers NG2, PDGFRβ, and fibronectin, with notably lower αSMA, CD146, P4H and desmin, and higher Col-IV expression. Nuclear NF-kB translocation in response to IL-1β stimulation was observed in both cultures, however, P-pericytes displayed elevated expression of the transcription factor C/EBPδ, and lower expression of the adhesion molecule ICAM-1. P-pericytes displayed elevated phagocytic and migratory ability. Both cultures responded similarly to stimulation by the growth factors TGFβ1 and PDGF-BB. Despite differences in their phenotype and magnitude of response, both P-pericytes and D-pericytes responded similarly to all examined functions, indicating that the neuroinflammatory phenotype of these cells is robust to culture conditions.
机译：脑周细胞会吞噬内皮，并有助于形成和维持血脑屏障。另外，周细胞参与CNS免疫应答的多个方面，包括瘢痕形成，粘附分子表达，趋化因子分泌和吞噬作用。通常使用体外培养物来研究脑周细胞的这些功能，但是，它们是高度可塑性的细胞，并且根据其培养条件可以显示出不同的表型和功能性反应。在这里，我们试图研究两种常用的培养基，即含有DMEM / F12的高血清和含有周细胞培养基（ScienCell）的低血清如何改变人脑周细胞的表型和神经炎症反应。从成年人脑活检组织中分离出周细胞，并在DMEM / F12（D-周细胞）或周细胞培养基（P-周细胞）中培养。免疫细胞化学，qRT-PCR和EdU掺入用于确定这如何改变其基础表型，包括周细胞标志物的表达，增殖和细胞形态。为了确定培养基是否改变了人脑周细胞的炎症反应，使用了免疫细胞化学，qRT-PCR，流式细胞仪和流式细胞仪来研究转录因子的诱导，趋化因子的分泌，粘附分子的表达，迁移，吞噬作用以及对炎症的反应相关的生长因子。与D-周细胞相比，P-周细胞显示出升高的增殖和明显的双极形态。另外，P-周细胞显示出与周细胞相关的标记NG2，PDGFRβ和纤连蛋白的较低表达，尤其是较低的αSMA，CD146，P4H和结蛋白，以及较高的Col-IV表达。在两种培养物中均观察到响应于IL-1β刺激的核NF-kB易位，但是P-周细胞显示出转录因子C /EBPδ的表达升高，而粘附分子ICAM-1的表达则较低。 P-周细胞显示出吞噬和迁移能力升高。两种培养物对生长因子TGFβ1和PDGF-BB的刺激反应相似。尽管它们的表型和应答程度不同，P-周细胞和D-周细胞对所有检查的功能都具有相似的反应，表明这些细胞的神经炎性表型对培养条件很强。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号