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首页> 外文期刊>BMC Physiology >The effect of marathon on mRNA expression of anti-apoptotic and pro-apoptotic proteins and sirtuins family in male recreational long-distance runners
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The effect of marathon on mRNA expression of anti-apoptotic and pro-apoptotic proteins and sirtuins family in male recreational long-distance runners

机译：马拉松对男子休闲长跑运动员抗凋亡和促凋亡蛋白及sirtuins家族mRNA表达的影响

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Background A large body of evidence shows that a single bout of strenuous exercise induces oxidative stress in circulating human lymphocytes leading to lipid peroxidation, DNA damage, mitochondrial perturbations, and protein oxidation. In our research, we investigated the effect of physical load on the extent of apoptosis in primary cells derived from blood samples of sixteen healthy amateur runners after marathon (a.m.). Results Blood samples were collected from ten healthy amateur runners peripheral blood mononuclear cells (PBMCs) were isolated from whole blood and bcl-2, bax, heat shock protein (HSP)70, Cu-Zn superoxide dismutase (SOD), Mn-SOD, inducible nitric oxide synthase (i-NOS), SIRT1, SIRT3 and SIRT4 (Sirtuins) RNA levels were determined by Northern Blot analysis. Strenuous physical load significantly increased HSP70, HSP32, Mn-SOD, Cu-Zn SOD, iNOS, GADD45, bcl-2, forkhead box O (FOXO3A) and SIRT1 expression after the marathon, while decreasing bax, SIRT3 and SIRT4 expression (P < 0.0001). Conclusion These data suggest that the physiological load imposed in amateur runners during marathon attenuates the extent of apoptosis and may interfere with sirtuin expression.
机译：背景技术大量证据表明，一次剧烈运动会在循环的人类淋巴细胞中诱导氧化应激，从而导致脂质过氧化，DNA损伤，线粒体微扰和蛋白质氧化。在我们的研究中，我们研究了体力负荷对马拉松（上午）后16位健康业余跑步者血液样本中原代细胞凋亡程度的影响。结果从全血中分离出十个健康的业余跑步运动员的外周血单个核细胞（PBMC），并从bcl-2，bax，热休克蛋白（HSP）70，Cu-Zn超氧化物歧化酶（SOD），Mn-SOD，诱导型一氧化氮合酶（i-NOS），SIRT1，SIRT3和SIRT4（Sirtuins）RNA水平通过Northern Blot分析确定。剧烈的物理负荷在马拉松后显着增加了HSP70，HSP32，Mn-SOD，Cu-Zn SOD，iNOS，GADD45，bcl-2，叉头盒O（FOXO3A）和SIRT1的表达，同时降低了bax，SIRT3和SIRT4的表达（P < 0.0001）。结论这些数据表明，马拉松运动员在业余跑步者身上施加的生理负荷减弱了细胞凋亡的程度，并可能干扰sirtuin的表达。

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