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首页> 外文期刊>BMC Plant Biology >Expression of human dopamine receptor in potato ( Solanum tuberosum ) results in altered tuber carbon metabolism
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Expression of human dopamine receptor in potato ( Solanum tuberosum ) results in altered tuber carbon metabolism

机译：人多巴胺受体在马铃薯中的表达导致块茎碳代谢的改变

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Background Even though the catecholamines (dopamine, norepinephrine and epinephrine) have been detected in plants their role is poorly documented. Correlations between norepinephrine, soluble sugars and starch concentration have been recently reported for potato plants over-expressing tyrosine decarboxylase, the enzyme mediating the first step of catecholamine synthesis. More recently norepinephrine level was shown to significantly increase after osmotic stress, abscisic acid treatment and wounding. Therefore, it is possible that catecholamines might play a role in plant stress responses by modulating primary carbon metabolism, possibly by a mechanism similar to that in animal cells. Since to date no catecholamine receptor has been identified in plants we transformed potato plants with a cDNA encoding human dopamine receptor (HD1). Results Tuber analysis of transgenic plants revealed changes in the activities of key enzymes mediating sucrose to starch conversion (ADP-glucose phosphorylase and sucrose synthase) and sucrose synthesis (sucrose phosphate synthase) leading to altered content of both soluble sugars and starch. Surprisingly the catecholamine level measured in transgenic plants was significantly increased; the reason for this is as yet unknown. However the presence of the receptor affected a broader range of enzyme activities than those affected by the massive accumulation of norepinephrine reported for plants over-expressing tyrosine decarboxylase. Therefore, it is suggested that the presence of the exogenous receptor activates catecholamine cAMP signalling in plants. Conclusions Our data support the possible involvement of catecholamines in regulating plant carbon metabolism via cAMP signalling pathway.
机译：背景技术即使已在植物中检测到儿茶酚胺（多巴胺，去甲肾上腺素和肾上腺素），其作用也文献不足。最近已报道过表达酪氨酸脱羧酶的马铃薯植物中去甲肾上腺素，可溶性糖和淀粉浓度之间的相关性，该酶介导儿茶酚胺合成的第一步。最近，在渗透压，脱落酸治疗和创伤后，去甲肾上腺素水平显着增加。因此，儿茶酚胺可能通过调节初级碳代谢而可能在植物胁迫反应中发挥作用，可能是通过类似于动物细胞的机制。迄今为止，我们尚未在植物中鉴定出儿茶酚胺受体，我们用编码人多巴胺受体（HD1）的cDNA转化了马铃薯植株。结果对转基因植物的块茎分析表明，介导蔗糖转化为淀粉的关键酶（ADP-葡萄糖磷酸化酶和蔗糖合酶）和蔗糖合成（蔗糖磷酸合酶）的活性发生了变化，导致可溶性糖和淀粉含量的改变。令人惊讶的是，在转基因植物中检测到的儿茶酚胺水平显着增加;原因尚不清楚。然而，与报道过过量表达酪氨酸脱羧酶的植物中去甲肾上腺素大量积累所影响的受体相比，该受体的存在对酶活性的影响范围更广。因此，建议外源受体的存在激活植物中的儿茶酚胺cAMP信号传导。结论我们的数据支持儿茶酚胺可能通过cAMP信号通路参与调节植物碳代谢。

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