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首页> 外文期刊>BMC Plant Biology >A combined linkage and regional association mapping validation and fine mapping of two major pleiotropic QTLs for seed weight and silique length in rapeseed (Brassica napus L.)
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A combined linkage and regional association mapping validation and fine mapping of two major pleiotropic QTLs for seed weight and silique length in rapeseed (Brassica napus L.)

机译：结合的连锁和区域关联图谱验证以及两个主要多效QTL的油菜籽（Brassica napus L.）种子重量和长角果长度的精细定位

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Background Seed weight (SW) and silique length (SL) are important determinants of the yield potential in rapeseed (Brassica napus L.). However, the genetic basis of both traits is poorly understood. The main objectives of this study were to dissect the genetic basis of SW and SL in rapeseed through the preliminary mapping of quantitative trait locus (QTL) by linkage analysis and fine mapping of the target major QTL by regional association analysis. Results Preliminary linkage mapping identified thirteen and nine consensus QTLs for SW and SL, respectively. These QTLs explained 0.7-67.1% and 2.1-54.4% of the phenotypic variance for SW and SL, respectively. Of these QTLs, three pairs of SW and SL QTLs were co-localized and integrated into three unique QTLs. In addition, the significance level and genetic effect of the three co-localized QTLs for both SW and SL showed great variation before and after the conditional analysis. Moreover, the allelic effects of the three QTLs for SW were highly consistent with those for SL. Two of the three co-localized QTLs, uq.A09-1 (mean R2?=?20.1% and 19.0% for SW and SL, respectively) and uq.A09-3 (mean R2?=?13.5% and 13.2% for SW and SL, respectively), were detected in all four environments and showed the opposite additive-effect direction. These QTLs were validated and fine mapped (their confidence intervals were narrowed down from 5.3?cM to 1?cM for uq.A09-1 and 13.2?cM to 2.5?cM for uq.A09-3) by regional association analysis with a panel of 576 inbred lines, which has a relatively rapid linkage disequilibrium decay (0.3?Mb) in the target QTL region. Conclusions A few QTLs with major effects and several QTLs with moderate effects might contribute to the natural variation of SW and SL in rapeseed. The meta-, conditional and allelic effect analyses suggested that pleiotropy, rather than tight linkage, was the genetic basis of the three pairs of co-localized of SW and SL QTLs. Regional association analysis was an effective and highly efficient strategy for the direct fine mapping of target major QTL identified by preliminary linkage mapping.
机译：背景种子重量（SW）和长角果长度（SL）是油菜（甘蓝型油菜）单产潜力的重要决定因素。然而，人们对这两个性状的遗传基础了解甚少。本研究的主要目的是通过连锁分析对数量性状基因座（QTL）进行初步定位，并通过区域关联分析对目标主要QTL进行精细定位，从而剖析油菜中SW和SL的遗传基础。结果初步连锁映射分别确定了SW和SL的13个和9个共有QTL。这些QTL分别解释了SW和SL的表型变异的0.7-67.1％和2.1-54.4％。在这些QTL中，三对SW和SL QTL被共定位并集成到三个独特的QTL中。此外，在条件分析前后，三个共定位的QTL对SW和SL的显着性水平和遗传效应均表现出很大的差异。此外，三个QTL对SW的等位基因效应与SL高度一致。三个共定位的QTL中的两个，即u09.a（平均值R 2 ？=？20.1％和19.0％，分别为SW和SL）和u09.a09-3（平均值R <在所有四个环境中均检测到sup> 2 ？= SW和SL的13.5％和13.2％），并显示出相反的加性效应方向。通过面板区域关联分析，对这些QTL进行了验证和精细映射（对A09-1的置信区间从5.3？cM缩小到1？cM，对于A09-3的置信区间从13.2？cM缩小到2.5？cM）。的576个自交系，在目标QTL区域具有相对快速的连锁不平衡衰减（0.3？Mb）。结论几个主要影响的QTL和几个中等影响的QTL可能会影响油菜SW和SL的自然变异。荟萃，条件和等位基因效应分析表明，多效性而不是紧密连锁是SW和SL QTL的三对共定位的遗传基础。区域关联分析是一种有效且高效的策略，可用于通过初步连锁映射确定的目标主要QTL的直接精细映射。

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