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首页> 外文期刊>Scientific reports. >Linkage and regional association analysis reveal two new tightly-linked major-QTLs for pod number and seed number per pod in rapeseed (Brassica napus L.)
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Linkage and regional association analysis reveal two new tightly-linked major-QTLs for pod number and seed number per pod in rapeseed (Brassica napus L.)

机译：连锁关系和区域关联分析揭示了两个新的紧密连锁的主QTL，分别针对油菜籽（ Brassica napus L。）中的荚数和每个荚的种子数。

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To facilitate the pseudochromosomes assembly and gene cloning in rapeseed, we developed a reference genetic population/map (named BnaZNF2) from two sequenced cultivars, Zhongshuang11 and No.73290, those exhibit significant differences in many traits, particularly yield components. The BnaZNF2 genetic map exhibited perfect collinearity with the physical map of B. napus , indicating its high quality. Comparative mapping revealed several genomic rearrangements between B. napus and B. rapa or B. oleracea . A total of eight and 16 QTLs were identified for pod number and seed number per pod, respectively, and of which three and five QTLs are identical to previously identified ones, whereas the other five and 11 are novel. Two new major QTL respectively for pod number and seed number per pod, qPN.A06-1 and qSN.A06-1 ( R 2?=?22.8% and 32.1%), were colocalised with opposite effects, and only qPN.A06-1 was confirmed and narrowed by regional association analysis to 180?kb including only 33 annotated genes. Conditional QTL analysis and subsequent NILs test indicated that tight linkage, rather than pleiotropy, was the genetic causation of their colocalisation. Our study demonstrates potential of this reference genetic population/map for precise QTL mapping and as a base for positional gene cloning in rapeseed.
机译：为了促进油菜中假染色体的组装和基因克隆，我们从中双11和73290两个测序品种中开发了一个参考遗传种群/图谱（命名为BnaZNF 2 ），它们在许多性状上表现出显着差异，特别是屈服分量。 BnaZNF 2 遗传图谱与油菜的物理图谱具有很好的共线性，表明其高质量。比较图谱揭示了甘蓝型油菜与甘蓝型油菜或甘蓝型油菜之间的几个基因组重排。总共确定了八个和16个QTL用于每个荚的荚数和种子数，其中三个和五个QTL与先前确定的相同，而其他五个和11个是新颖的。两个新的主要荚果数量和每个荚果的种子数量的最大QTL qPN.A06-1和qSN.A06-1（R 2？ =？22.8％和32.1％）被共定位有相反的效果，并且只有qPN.A06-1被证实，并通过区域关联分析缩小到180？kb，仅包含33个带注释的基因。有条件的QTL分析和随后的NILs测试表明紧密联系而不是多效性是其共定位的遗传原因。我们的研究表明该参考遗传群体/图谱可用于精确的QTL定位，并可作为油菜中位置基因克隆的基础。

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