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首页> 外文期刊>Brazilian Journal of Microbiology >Production of xylanase and CMCase on solid state fermentation in different residues by Thermoascus aurantiacus miehe
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Production of xylanase and CMCase on solid state fermentation in different residues by Thermoascus aurantiacus miehe

机译：米色热曲霉固态发酵在不同残渣中产生木聚糖酶和CMCase

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摘要

The use of waste as raw material is important for government economy and natural balance. The purpose of this work was to study the production of CMCase and xylanase by a Brazilian strain of Thermoascus aurantiacus in solid state fermentation (SSF) using different agricultural residues (wheat bran, sugarcane bagasse, orange bagasse, corncob, green grass, dried grass, sawdust and corn straw) as substrates without enrichment of the medium and characterize the crude enzymes.The study of the extracellular cellulolytic and hemicellulolytic enzymes showed that T. arantiacus is more xylanolytic than cellulolytic. The highest levels of enzymes were produced in corncob, grasses and corn straw. All the enzymes were stable at room temperature by 24 h over a broad pH range (3.0-9.0) and also were stable at 60oC for 1 h. The optimum pH and temperature for xylanase and CMCase were 5.0-5.5 and 5.0 and 75oC, respectively. The microorganism grew quickly in stationary, simple and low cost medium. The secreted extracellular enzymes presented properties that match with those frequently required in industrial environment.
机译：使用废物作为原材料对于政府经济和自然平衡很重要。这项工作的目的是研究使用不同的农业残留物（麦麸，甘蔗渣，橙渣，玉米芯，绿草，干草，木屑和玉米秸秆）作为底物而没有富集培养基并表征粗酶。对细胞外纤维素分解酶和半纤维素分解酶的研究表明，Arantiacus的木聚糖分解度比纤维素分解度高。玉米芯，草和玉米秸秆中产生的酶水平最高。在室温下，在宽pH范围（3.0-9.0）下24小时所有酶都稳定，并且在60°C稳定1小时。木聚糖酶和CMCase的最佳pH和温度分别为5.0-5.5和5.0和75oC。微生物在固定，简单和低成本的培养基中快速生长。分泌的细胞外酶具有与工业环境中经常需要的那些特性相匹配的特性。

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