利用产木聚糖酶的黑曲霉(Aspergillus niger)GXH-1菌株对木薯渣和麸皮发酵,通过单因素和响应面试验优化产酶条件.最佳发酵条件为15.99%的木薯渣与84.01%的麸皮组成发酵基料;以1.60%的(NH4)2 SO4为氮源;含水量为56.27%,KH2 PO4和NaOH质量分数分别为0.12%和0.20%,接种量为4.0%;36.23℃ 发酵68 h.在此条件下,木聚糖酶酶活力最高为1258.74 U/g,是优化前的3.26倍,优化效果显著.%The cassava dregs and bran were fermented by Aspergillus niger GXH-1 strain to produce xylanase.Xylanase production conditions were optimized by single factor and response surface experiments.The optimum conditions for producing xylanase were as follows:the composition of culture medium was 15.99% cassava dregs and 84.01% bran,the nitrogen source was 1.60% (NH4 )2 SO4 ,the water content was 56.27%,the content of KH 2 PO4 andN aOH were 0.12% and 0.20%,respec-tively,the inoculum was 4.0%,the fermentation temperature was 36.23℃and the time was 68 h.Under the conditions,the enzyme activity of xylanase produced reach 1258.74 U/g,which was 3.26 times higher than that before optimization.
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