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首页> 外文期刊>Canadian Journal of Pure and Applied Sciences >A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY OF ACTIVE COMPONENT IN PARACETAMOL SYRUP CONTAMINATED BY PENICILLIUM EXPANSUM.
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A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY OF ACTIVE COMPONENT IN PARACETAMOL SYRUP CONTAMINATED BY PENICILLIUM EXPANSUM.

机译：高效液相色谱法测定青霉菌素污染的帕拉他莫糖浆中活性成分的含量。

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In this study, a high performance liquid chromatographic assay was carried out at room temperature to determine the concentration of active ingredient in paracetamol syrup contaminated by Penicillium expansum. The drug samples were extracted in methanol while the mobile phase was methanol: water (20:80 v / v ) mixture. A volume of 20ul of various concentrations of reference standard (4-aminophenol), contaminated and non-contaminated paracetamol syrups were injected separately in triplicate into a C 18 analytical column under reverse phase chromatographic conditions. The mean peak height, peak area and retention time for the contaminated sample were 3.47mUA, 23.58mUA 2 and 3.51minutes, whereas the non contaminated sample had an average peak height of 368.53mUA, peak area of 2470.04mUA 2 and retention time of 3.13 minutes. The standard curve generated from the data yielded perfect linearity with correlation coefficient, r=0.9990. The concentration of paracetamol in the contaminated sample was 0.36ug/ml while the control had 37.53ug/ml. The active component in the contaminated Paracetamol syrup was completely metabolized and transformed into a metabolite with an averag e peak area of 300.55mUA 2 , peak height of 20.05mUA and retention time of 3.15 minutes, by Penicillium expansum. These results confirm that Penicillium expansum can degrade some pharmaceutical drugs and transform them to unrelated forms.
机译：在这项研究中，在室温下进行了高效液相色谱测定，以确定被扩展的青霉菌污染的扑热息痛糖浆中活性成分的浓度。药物样品在甲醇中萃取，而流动相为甲醇：水（20:80 v / v）混合物。在反相色谱条件下，一式三份将20ul体积的各种浓度的参考标准品（4-氨基苯酚），被污染的和未污染的扑热息痛糖浆分别一式三份注入C 18分析柱中。被污染样品的平均峰高，峰面积和保留时间分别为3.47mUA，23.58mUA 2和3.51分钟，而未污染样品的平均峰高为368.53mUA，峰面积为2470.04mUA 2和保留时间为3.13分钟。分钟。从数据生成的标准曲线产生了具有相关系数r = 0.9990的完美线性。污染样品中对乙酰氨基酚的浓度为0.36ug / ml，而对照的浓度为37.53ug / ml。被污染的扑热息痛糖浆中的活性成分被青霉完全代谢并转化为平均峰面积为300.55mUA 2，峰高为20.05mUA且保留时间为3.15分钟的代谢物。这些结果证实，扩张青霉可以降解某些药物并将其转化为不相关的形式。

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