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首页> 外文期刊>Canadian Journal of Pure and Applied Sciences >SESSILE AEROBIC MICROBIOTA FROM THE WALL OF THE NATIONAL MUSEUM, BRAZIL: CHARACTERIZATION AND QUANTIFICATION
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SESSILE AEROBIC MICROBIOTA FROM THE WALL OF THE NATIONAL MUSEUM, BRAZIL: CHARACTERIZATION AND QUANTIFICATION

机译：来自巴西国家博物馆壁的无氧微生物菌：表征和定量

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摘要

The interest in preserving cultural heritage sites and artifacts has driven the development of additional measures to protect them from deterioration, which increases over time. The Garden of the Princess (Jardim da Princesa) located at the National Museum in Rio de Janeiro, Brazil, was the main site under evaluatio n in this work. Microbial colonizatio n of the garden's walls was evaluated at two different areas by cultivation -based approach using specific media for fungi and bacteria, including total heterotrophic bacteria, acid and iron-producing bacteria. Results demonstrated that the higher cell density was detected in the sample fro m the higher humidity despite the limited humidity of the walls. Molecular identification o f isolates revealed that Arthrobacter was the main bacterial genus in the biofilm, even though the others predominant genera have longer survival times during starvatio n and higher resistance to desiccation. Sessile fungi, despite fewer in number, were also quite diverse. In conclusion, even found in such hostile environment an interesting microbial diversity was observed in this study including four genera not yet reported as biodeterioration agents in heritage sites: bacteria Ensifer, Enterobacter and Srenotrophomonas and the fungus Hypocreacea.
机译：对保护文化遗产和文物的兴趣推动了其他措施的发展，以保护它们免遭破坏，而随着时间的流逝，这种恶化会增加。位于这项工作的主要地点是位于巴西里约热内卢国家博物馆的公主花园（Jardim da Princesa）。通过使用基于真菌和细菌（包括总异养细菌，产酸和生铁细菌）的特定培养基的栽培方法，在两个不同的区域对花园墙壁的微生物定植进行了评估。结果表明，尽管壁的湿度有限，但在较高的湿度下仍可在样品中检测到较高的细胞密度。分离株的分子鉴定表明，节杆菌是生物膜中的主要细菌属，即使其他主要属在饥饿期间具有更长的生存时间和更高的抗干燥性。无柄真菌尽管数量较少，但也很多样化。总之，即使在如此恶劣的环境中也发现了有趣的微生物多样性，包括尚未报道为遗产地生物降解剂的四个属：Ensifer，Enterobacter和Srenotrophomonas细菌和真菌性菌cre。

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