ISOLATION, CHARACTERIZATION, AND BIOACTIVITY OF DEEP SEA BACTERIA WITH SPECIAL REFERENCE TO INDUCTION OF ANTIBACTERIAL AND ANTIOXIDANT METABOLITES FOLLOWING GAMMA IRRADIATION

Origanti D Rakesh; Rajiv Pathak; Atlar Singh Dhaker; Rajesh Arora; Raj Kumar; R. Rajaram; Hemant K Gautam

首页> 外文期刊>Canadian Journal of Pure and Applied Sciences >ISOLATION, CHARACTERIZATION, AND BIOACTIVITY OF DEEP SEA BACTERIA WITH SPECIAL REFERENCE TO INDUCTION OF ANTIBACTERIAL AND ANTIOXIDANT METABOLITES FOLLOWING GAMMA IRRADIATION

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ISOLATION, CHARACTERIZATION, AND BIOACTIVITY OF DEEP SEA BACTERIA WITH SPECIAL REFERENCE TO INDUCTION OF ANTIBACTERIAL AND ANTIOXIDANT METABOLITES FOLLOWING GAMMA IRRADIATION

机译：深海细菌的分离，表征和生物活性，特别涉及在γ射线辐照后诱导抗菌和抗氧化剂代谢

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With an objective to find new marine-derived antibiotics and antioxidants, marine bacteria were isolated from sediment samples collected from depths of 556 m and 698 m in Laccadive Sea of Indian Ocean. The antibacterial and antioxidant activity of these bacteria was studied. Preliminary assays revealed that none of the isolates possess antibacterial activity. However, exposure to gamma radiation resulted in synthesis of metabolites in one of the strain leading to the observation of antibacterial effect. Parallel to this, all the strains were evaluated for their antioxidant activity. The same strain, which exhibited antibacterial activity after gamma-irradiation, showed potent antioxidant activity. The extract which showed biological activity was studied by thin layer chromatography studies. The isolated strain was characterised to be Bacillus sonorensis based on phylogenetic analyses using molecular biological techniques.

机译：为了找到新的海洋来源的抗生素和抗氧化剂，从印度洋拉卡迪奇海的556 m和698 m深度收集的沉积物中分离出海洋细菌。研究了这些细菌的抗菌和抗氧化活性。初步分析显示，这些分离株均不具有抗菌活性。然而，暴露于γ射线导致一种菌株中代谢产物的合成，导致观察到抗菌作用。与此平行，评估所有菌株的抗氧化活性。在伽玛射线辐照后显示出抗菌活性的同一菌株显示出强大的抗氧化活性。通过薄层色谱法研究了具有生物活性的提取物。基于使用分子生物学技术的系统发育分析，分离的菌株被表征为sonorensis。

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《Canadian Journal of Pure and Applied Sciences》 |2011年第1期|共8页
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Origanti D Rakesh; Rajiv Pathak; Atlar Singh Dhaker; Rajesh Arora; Raj Kumar; R. Rajaram; Hemant K Gautam;
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ISOLATION, CHARACTERIZATION, AND BIOACTIVITY OF DEEP SEA BACTERIA WITH SPECIAL REFERENCE TO INDUCTION OF ANTIBACTERIAL AND ANTIOXIDANT METABOLITES FOLLOWING GAMMA IRRADIATION

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