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首页> 外文期刊>Cancer Cell International >Promoter methylation status of the tumor suppressor gene SOX11 is associated with cell growth and invasion in nasopharyngeal carcinoma
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Promoter methylation status of the tumor suppressor gene SOX11 is associated with cell growth and invasion in nasopharyngeal carcinoma

机译：抑癌基因SOX11的启动子甲基化状态与鼻咽癌的细胞生长和侵袭有关

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The transcription factor SOX11 is one of members of the SRY box-containing (SOX) family emerging as important transcriptional regulators. In recent years, up-regulation of SOX11 has been detected in various types of solid tumors. In this study, the effects of promoter methylation of the SOX11 gene on SOX11 expression and cell growth and invasion of nasopharyngeal carcinoma were investigated. In this study,methylation-specific PCR and real time quantitative PCR have been applied to investigate the effect of promoter methylation of the SOX11 gene on SOX11 expression in the nasopharyngeal carcinoma and chronic inflammation tissues. The nasopharyngeal carcinoma cell line (CNE2) was treated with 5-aza-2'-deoxycytidine. The effect of promoter methylation of SOX11 on growth and invasion of nasopharyngeal carcinoma cells was detected with MTT test and Boyden chamber Matrigel invasion assay. No or weak expression of SOX11 mRNA was detected in the nasopharyngeal carcinoma tissues of SOX11 gene promoter methylation. Strong expression of SOX11 mRNA was detected in the nasopharyngeal carcinoma tissues of SOX11 gene promoter unmethylation and chronic inflammation tissues of pharynx nasalis. SOX11 mRNA and protein were re-expressed, SOX11 gene was demethylated, and growth and invasion of cells were inhibited in CNE2 cell line after 5-aza-2'-deoxycytidine treatment. The results of the study indicate that expression of SOX11 mRNA and protein were related to SOX11 gene methylation status. SOX11 gene methylation may be plays a role in growth and invasion of nasopharyngeal carcinoma cells.
机译：转录因子SOX11是包含SRY盒（SOX）的家族之一，成为重要的转录调节因子。近年来，已在各种类型的实体瘤中检测到SOX11的上调。在这项研究中，研究了SOX11基因的启动子甲基化对鼻咽癌中SOX11表达以及细胞生长和侵袭的影响。在这项研究中，甲基化特异性PCR和实时定量PCR已用于研究SOX11基因启动子甲基化对鼻咽癌和慢性炎症组织中SOX11表达的影响。用5-氮杂-2'-脱氧胞苷处理鼻咽癌细胞系（CNE2）。用MTT试验和Boyden室基质胶侵袭试验检测SOX11启动子甲基化对鼻咽癌细胞生长和侵袭的影响。在鼻咽癌组织中未检测到SOX11基因启动子甲基化或表达较弱。在SOX11基因启动子非甲基化的鼻咽癌组织和咽鼻的慢性炎症组织中检测到SOX11 mRNA的强表达。在5-氮杂-2'-脱氧胞苷处理后，CNE2细胞系中SOX11 mRNA和蛋白重新表达，SOX11基因去甲基化，并且抑制了细胞的生长和侵袭。研究结果表明，SOX11 mRNA和蛋白的表达与SOX11基因甲基化状态有关。 SOX11基因甲基化可能在鼻咽癌细胞的生长和侵袭中起作用。

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