BackgroundThe breed-defining dorsal ridge in Rhodesian Ridgeback dogs is the result of a 133,000 base pair duplication on chromosome 18. Because this trait is dominant, heterozygous dogs cannot be discriminated from those with two copies of the Ridge allele. ResultsA quantitative PCR test was developed and dogs of known genotype were used as test subjects. In all cases, the correct genotype was determined experimentally. ConclusionsThis work provides a rapid and accurate methodology for determining dog genotype with respect to the Ridge allele.
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