• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Canine Genetics and Epidemiology >Analysis of DLA-DQB1 and polymorphisms in CTLA4 in Cocker spaniels affected with immune-mediated haemolytic anaemia
【24h】

Analysis of DLA-DQB1 and polymorphisms in CTLA4 in Cocker spaniels affected with immune-mediated haemolytic anaemia

机译：免疫介导的溶血性贫血的可卡犬的DLA-DQB1和CTLA4多态性分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BackgroundCocker spaniels are predisposed to immune-mediated haemolytic anaemia (IMHA), suggesting that genetic factors influence disease susceptibility. Dog leukocyte antigen (DLA) class II genes encode major histocompatibility complex (MHC) molecules that are involved in antigen presentation to CD4+ T cells. Several DLA haplotypes have been associated with autoimmune disease, including IMHA, in dogs, and breed specific differences have been identified. Cytotoxic T lymphocyte antigen 4 (CTLA4) is a critical molecule involved in the regulation of T-cell responses. Single nucleotide polymorphisms (SNPs) in the CTLA4 promoter have been shown to be associated with several autoimmune diseases in humans and more recently with diabetes mellitus and hypoadrenocorticism in dogs. The aim of the present study was to investigate whether DLA-DQB1 alleles or CTLA4 promoter variability are associated with risk of IMHA in Cocker spaniels. ResultsThere were a restricted number of DLA-DQB1 alleles identified, with a high prevalence of DLA-DQB1*007:01 in both groups. A high prevalence of DLA-DQB1 homozygosity was identified, although there was no significant difference between IMHA cases and controls. CTLA4 promoter haplotype diversity was limited in Cocker spaniels, with all dogs expressing at least one copy of haplotype 8. There was no significant difference comparing haplotypes in the IMHA affected group versus control group (p?=?0.23). Homozygosity for haplotype 8 was common in Cocker spaniels with IMHA (27/29; 93?%) and in controls (52/63; 83?%), with no statistically significant difference in prevalence between the two groups (p?=?0.22). ConclusionsDLA-DQB1 allele and CTLA4 promoter haplotype were not found to be significantly associated with IMHA in Cocker spaniels. Homozygosity for DLA-DQB1*007:01 and the presence of CTLA4 haplotype 8 in Cocker spaniels might increase overall susceptibility to IMHA in this breed, with other genetic and environmental factors involved in disease expression and progression.
机译：背景可卡犬易患免疫介导的溶血性贫血（IMHA），表明遗传因素影响疾病的易感性。狗白细胞抗原（DLA）II类基因编码主要的组织相容性复合体（MHC）分子，这些分子参与抗原呈递给CD4 + T细胞。犬中有几种DLA单倍型与自身免疫性疾病（包括IMHA）有关，并且已经确定了品种特异性差异。细胞毒性T淋巴细胞抗原4（CTLA4）是参与T细胞反应调节的关键分子。 CTLA4启动子中的单核苷酸多态性（SNP）已显示与人类的几种自身免疫性疾病有关，最近与狗的糖尿病和肾上腺皮质功能低下有关。本研究的目的是调查可卡犬中的DLA-DQB1等位基因或CTLA4启动子变异性是否与IMHA风险相关。结果鉴定出的DLA-DQB1等位基因数量有限，两组中DLA-DQB1 * 007：01的患病率很高。尽管IMHA病例与对照之间没有显着差异，但DLA-DQB1纯合子的流行率很高。在可卡犬中，CTLA4启动子的单倍型多样性受到限制，所有的狗都表达至少一个单倍型8。在IMHA感染组与对照组中，比较单倍型没有显着差异（p≥0.23）。在具有IMHA的可卡犬（27/29; 93％）和对照组（52/63; 83％）中，单倍型8的纯合性很常见，两组之间的患病率无统计学差异（p == 0.22） ）。结论在可卡犬中未发现DLA-DQB1等位基因和CTLA4启动子单倍型与IMHA显着相关。可卡犬的DLA-DQB1 * 007：01纯合性和CTLA4单倍型8的存在可能会增加该品种对IMHA的总体易感性，其他遗传和环境因素也会影响疾病的表达和进展。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号