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High-yield production of extracellular type-I cellulose by the cyanobacterium Synechococcus sp. PCC 7002

机译:蓝细菌 Synechococcus sp。高产细胞外I型纤维素。 PCC 7002

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Cellulose synthase, encoded by the cesA gene, is responsible for the synthesis of cellulose in nature. We show that the cell wall of the cyanobacterium Synechococcus sp. PCC 7002 naturally contains cellulose. Cellulose occurs as a possibly laminated layer between the inner and outer membrane, as well as being an important component of the extracellular glycocalyx in this cyanobacterium. Overexpression of six genes, cmc–ccp–cesAB–cesC–cesD–bgl, from Gluconacetobacter xylinus in Synechococcus sp. PCC 7002 resulted in very high-yield production of extracellular type-I cellulose. High-level cellulose production only occurred when the native cesA gene was inactivated and when cells were grown at low salinity. This system provides a method for the production of lignin-free cellulose from sunlight and CO2 for biofuel production and other biotechnological applications.
机译:cesA基因编码的纤维素合酶负责自然界中纤维素的合成。我们表明,蓝藻Synechococcus sp的细胞壁。 PCC 7002自然包含纤维素。纤维素是内膜和外膜之间可能的层压层,也是该蓝细菌中细胞外糖萼的重要成分。来自Synochoococcus sp。的木糖葡糖杆菌的六个基因,cmc-ccp-cesAB-cesC-cesD-bgl的过表达。 PCC 7002导致细胞外I型纤维素的高产量生产。仅当天然cesA基因失活并且细胞在低盐度下生长时,才发生高水平的纤维素生产。该系统提供了一种从日光和CO 2 中生产不含木质素的纤维素的方法,用于生物燃料生产和其他生物技术应用。

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