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首页> 外文期刊>Marine Drugs >Biogenic Polyphosphate Nanoparticles from a Marine Cyanobacterium Synechococcus sp. PCC 7002: Production, Characterization, and Anti-Inflammatory Properties In Vitro
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Biogenic Polyphosphate Nanoparticles from a Marine Cyanobacterium Synechococcus sp. PCC 7002: Production, Characterization, and Anti-Inflammatory Properties In Vitro

机译：来自海洋蓝细菌Syechococcus sp。的生物多磷酸盐纳米颗粒。 PCC 7002：体外生产，表征和抗炎特性

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Probiotic-derived polyphosphates have attracted interest as potential therapeutic agents to improve intestinal health. The current study discovered the intracellular accumulation of polyphosphates in a marine cyanobacterium Synechococcus sp. PCC 7002 as nano-sized granules. The maximum accumulation of polyphosphates in Synechococcus sp. PCC 7002 was found at the late logarithmic growth phase when the medium contained 0.74 mM of KH 2 PO 4 , 11.76 mM of NaNO 3 , and 30.42 mM of Na 2 SO 4 . Biogenic polyphosphate nanoparticles (BPNPs) were obtained intact from the algae cells by hot water extraction, and were purified to remove the organic impurities by Sephadex G-100 gel filtration. By using 100 kDa ultrafiltration, BPNPs were fractionated into the larger and smaller populations with diameters ranging between 30–70 nm and 10–30 nm, respectively. 4′,6-diamidino-2-phenylindole fluorescence and orthophosphate production revealed that a minor portion of BPNPs (about 14–18%) were degraded during simulated gastrointestinal digestion. In vitro studies using lipopolysaccharide-activated RAW264.7 cells showed that BPNPs inhibited cyclooxygenase-2, inducible nitric oxide (NO) synthase expression, and the production of proinflammatory mediators, including NO, tumor necrosis factor-α, interleukin-6, and interleukin-1β through suppressing the Toll-like receptor 4/NF-κB signaling pathway. Overall, there is promise in the use of the marine cyanobacterium Synechococcus sp. PCC 7002 to produce BPNPs, an anti-inflammatory postbiotic.
机译：益生菌衍生的多磷酸盐作为改善肠道健康的潜在治疗剂已引起人们的兴趣。目前的研究发现了海洋蓝藻Syechococcus sp。中多磷酸盐的细胞内积累。 PCC 7002为纳米颗粒。 Synechococcus sp。中多磷酸盐的最大积累。 PCC 7002被发现在对数生长期后期，该培养基含有0.74 mM的KH 2 PO 4，11.76 mM的NaNO 3和30.42 mM的Na 2 SO 4。通过热水提取从藻类细胞中完整获得生物多磷酸盐纳米颗粒（BPNPs），并通过Sephadex G-100凝胶过滤纯化以去除有机杂质。通过使用100 kDa超滤，将BPNPs分为较大和较小的种群，直径分别在30–70 nm和10–30 nm之间。 4'，6-二mid基-2-苯基吲哚的荧光和正磷酸盐的产生表明，在模拟胃肠消化过程中，一小部分BPNP（约14-18％）被降解。使用脂多糖激活的RAW264.7细胞进行的体外研究表明BPNP抑制了环氧合酶2，诱导型一氧化氮（NO）合酶的表达以及促炎性介质的产生，包括NO，肿瘤坏死因子α，白介素6和白介素。 -1β通过抑制Toll样受体4 /NF-κB信号通路。总体而言，海洋蓝藻Synechococcus sp。的使用前景广阔。 PCC 7002产生抗炎的后生物BPNPs。

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