• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell Health and Cytoskeleton >Cell surface area regulation in neurons in hippocampal slice cultures is resistant to oxygen-glucose deprivation
【24h】

Cell surface area regulation in neurons in hippocampal slice cultures is resistant to oxygen-glucose deprivation

机译：海马切片培养物中神经元的细胞表面积调节可抵抗氧葡萄糖剥夺

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Neurons swell in response to a variety of insults. The capacity to recover, ie, to shrink, is critical for neuronal function and survival. Studies on dissociated neurons have shown that during swelling and shrinking, neurons reorganize their plasma membrane; as neurons swell, in response to hypo-osmotic media, the bilayer area increases. Upon restoration of normo-osmotic media, neurons shrink, forming transient invaginations of the plasma membrane known as vacuole-like dilations (VLDs), to accommodate the decrease in the bilayer.Methods: Here we used confocal microscopy to monitor neuronal swelling and shrinking in the three-dimensional (3D) environment of post-natal rat hippocampal slice cultures. To label neurons, we used biolistic transfection, to introduce enhanced green fluorescent protein (eGFP) targeted to the cytoplasm; and a membrane targeted GFP (lckGFP), targeted to the plasma membrane.Results: Neurons in slice cultures swelled and shrank in response to hypo-osmotic to normo-osmotic media changes. Oxygen-glucose deprivation (OGD) caused sustained neuronal swelling; after reperfusion, some neurons recovered but in others, VLD recovery was stalled. OGD did not impair neuronal capacity to recover from a subsequent osmotic challenge.Conclusion: These results suggest cell surface area regulation (SAR) is an intrinsic property of neurons, and that neuronal capacity for SAR may play an important role in the brain’s response to ischemic insults.
机译：背景：神经元因各种侮辱而肿胀。恢复（即收缩）的能力对于神经元功能和生存至关重要。对离体神经元的研究表明，在肿胀和萎缩期间，神经元会重组其质膜。随着神经元肿胀，响应低渗介质，双层面积增加。恢复正常渗透介质后，神经元收缩，形成质膜的短暂侵袭，称为液泡状扩张（VLD），以适应双层细胞的减少。方法：在这里，我们使用共聚焦显微镜来监测神经元的肿胀和收缩。出生后大鼠海马切片培养的三维（3D）环境。为了标记神经元，我们使用了生物弹射法转染，以引入针对细胞质的增强型绿色荧光蛋白（eGFP）;结果：切片培养物中的神经元响应于低渗至正渗介质的变化而膨胀和收缩。缺氧葡萄糖（OGD）引起持续的神经元肿胀;再灌注后，一些神经元恢复了，但在另一些神经元中，VLD恢复却停滞了。 OGD并未损害神经元从随后的渗透挑战中恢复的能力。结论：这些结果表明细胞表面积调节（SAR）是神经元的固有特性，并且SAR的神经元能力可能在大脑对缺血的反应中起重要作用侮辱。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号