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首页> 外文期刊>Cellular Oncology: Analytical Cellular Pathology >Diagnostic DNA-Flow- vs. -Image-Cytometry in Effusion Cytology
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Diagnostic DNA-Flow- vs. -Image-Cytometry in Effusion Cytology

机译：渗流细胞学中的诊断性DNA流与图像流式细胞术

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Aims: To determine the sensitivity and specificity of flow‐ and image‐cytometry for the detection of DNA‐aneuploidy as a marker for malignant cells in effusions.Methods: 200 effusions (80 tumor cell‐positive, 74 negative and 46 cytologically equivocal) were stained with DAPI‐SR for DNA‐flow‐ and with Feulgen‐Pararosaniline for ‐image‐cytometry. They were measured using a PAS‐flow‐cytometer and an AutoCyte‐QUIC‐DNA‐workstation according to the ESACP consensus reports for DNA‐flow‐ and ‐image‐cytometry, respectively [7,23,29,49].Results: Sensitivity of DNA‐aneuploidy for the identification of malignant cells was 32.1% for DNA‐flow‐ and 75.0% for ‐image‐cytometry, specificity of ‐euploidy in benign cells was 100.0% for both methods. Positive predictive value of DNA‐aneuploidy for the identification of malignant cells was 100.0% for both techniques, negative predictive value of DNA‐euploidy was 48.6% for DNA‐flow‐ and 72.0% for ‐image‐cytometry.Conclusions: Searching for DNA‐aneuploidy as a diagnostic marker for neoplastic cells in serous effusions image‐cytometry revealed superior sensitivity as compared with monoparametric flow cytometry.
机译：目的：确定流式细胞仪和图像流式细胞仪检测DNA非整倍性作为积液中恶性细胞标记的敏感性和特异性方法：200例积液（80例肿瘤细胞阳性，74例阴性和46例细胞学上不明确）用DAPI‐SR染色进行DNA流动染色，并用Feulgen‐Pararosaniline进行图像细胞计数染色。根据ESACP共识报告，分别使用PAS流式细胞仪和AutoCyte-QUIC-DNA工作站对DNA进行流式细胞术和图像流式细胞术测量[7,23,29,49]。结果：敏感性DNA-非整倍性用于鉴定恶性细胞的比例在DNA流动检测中为32.1％，在图像细胞计数法中为75.0％，两种方法在良性细胞中的整倍体特异性为100.0％。两种技术对DNA的非整倍性鉴定的阳性预测值均为100.0％，对于DNA流动的DNA整倍体检测的阴性预测值为48.6％，对于图像细胞计数法则为72.0％。与单参数流式细胞术相比，非整倍性作为浆液性积液中肿瘤细胞的诊断标志物显示出更高的敏感性。

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