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Microtubule-binding protein FOR20 promotes microtubule depolymerization and cell migration

机译：微管结合蛋白FOR20促进微管解聚和细胞迁移

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Microtubules are highly dynamic filaments assembled from αβ-tubulin heterodimers and play important roles in many cellular processes, including cell division and migration. Microtubule dynamics is tightly regulated by microtubule-associated proteins (MAPs) that function by binding to microtubules or free tubulin dimers. Here, we report that FOR20 (FOP-related protein of 20?kDa), a conserved protein critical for ciliogenesis and cell cycle progression, is a previously uncharacterized MAP that facilitates microtubule depolymerization and promotes cell migration. FOR20 not only directly binds to microtubules but also regulates microtubule dynamics in vitro by decreasing the microtubule growth rate and increasing the depolymerization rate and catastrophe frequency. In the in vitro microtubule dynamics assays, FOR20 appears to preferentially interact with free tubulin dimers over microtubules. Depletion of FOR20 inhibits microtubule depolymerization and promotes microtubule regrowth after the nocodazole treatment in HeLa cells. In addition, FOR20 knockdown significantly inhibits both individual and collective migration of mammalian cells. Taken together, these data suggest that FOR20 functions as a MAP to promote microtubule depolymerization and cell migration.
机译：微管是由αβ-微管蛋白异二聚体组装而成的高动态细丝，在许多细胞过程（包括细胞分裂和迁移）中起着重要作用。微管动力学受到微管相关蛋白（MAPs）的严格调控，该蛋白通过与微管或游离微管蛋白二聚体结合而起作用。在这里，我们报告说，FOR20（与FOP相关的蛋白20kkDa）对纤毛发生和细胞周期进程至关重要，是一种保守的蛋白，它以前是未表征的MAP，可促进微管解聚并促进细胞迁移。 FOR20不仅直接与微管结合，而且通过降低微管的生长速率和增加解聚速率和突变频率来调节体外的微管动力学。在体外微管动力学分析中，FOR20似乎比微管优先与游离微管蛋白二聚体相互作用。诺福达唑处理HeLa细胞后，FOR20的耗尽会抑制微管解聚并促进微管再生。此外，FOR20的组合大大抑制了哺乳动物细胞的个体和集体迁移。综上所述，这些数据表明FOR20充当MAP来促进微管解聚和细胞迁移。

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