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首页> 外文期刊>Catalysts >Production of Resveratrol by Piceid Deglycosylation Using Cellulase
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Production of Resveratrol by Piceid Deglycosylation Using Cellulase

机译：使用纤维素酶通过糖基去糖基化生产白藜芦醇

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Resveratrol is a dietary polyphenolic compound widely used in medicine, food, and cosmetic products. The glycoside form of resveratrol, piceid, is also present in several plant materials but is less bioavailable. In this study, enzymatic transformation of piceid into resveratrol using inexpensive cellulase was investigated. Response surface methodology was used to evaluate the effect of reaction parameters, including reaction temperature, reaction time, enzyme amount and pH. The optimal conditions for biotransformation of piceid to resveratrol are: a reaction temperature of 50 °C, reaction time of 4.75 h, enzyme amount of 2.5 fungal β-glucanase (FBG) units and pH of 4.3. In addition, the extracts from Polygonum cuspidatum root contained high amounts of piceid were treated with cellulase in order to deglycosylation that increased resveratrol yield. After treatment, the resveratrol yield significantly increased from 2.72 to 9.49 mg/g, while the piceid contents decreased from 8.60 to 0 mg/g. The result provides an efficient method to convert piceid in the extracts of P. cuspidatum root into resveratrol by cellulase.
机译：白藜芦醇是一种饮食中的多酚类化合物，广泛用于医药，食品和化妆品中。白藜芦醇的糖苷形式，piceid，也存在于几种植物材料中，但生物利用度较低。在这项研究中，研究了使用廉价的纤维素酶将木脂苷酶促转化为白藜芦醇。响应面法用于评估反应参数的影响，包括反应温度，反应时间，酶量和pH。仿生菌素向白藜芦醇生物转化的最佳条件是：反应温度为50°C，反应时间为4.75 h，酶量为2.5真菌β-葡聚糖酶（FBG）单位，pH为4.3。另外，用纤维素酶处理虎杖根中含有大量木虱的提取物，以进行去糖基化，从而增加白藜芦醇的产量。处理后，白藜芦醇的产量从2.72毫克/克显着增加到9.49毫克/克，而类木脂含量从8.60毫克/克降低到0毫克/克。该结果提供了一种有效的方法，通过纤维素酶将虎杖根提取物中的皂苷转化为白藜芦醇。

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