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首页> 外文期刊>Cellular Oncology: Analytical Cellular Pathology >Localization of ORC1 During the Cell Cycle in Human Leukemia Cells
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Localization of ORC1 During the Cell Cycle in Human Leukemia Cells

机译：ORC1在人类白血病细胞周期中的定位

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The interaction of the origin recognition complex (ORC) with replication origins is a critical parameter in eukaryotic replication initiation. In mammals the ORC remains bound except during mitosis, thus the localization of ORC complexes allows localization of origins. A monoclonal antibody that recognizes human ORC1 was used to localize ORC complexes in populations of human MOLT-4 cells separated by cell cycle position using centrifugal elutriation. ORC1 staining in cells in early G1 is diffuse and primarily peripheral. As the cells traverse G1, ORC1 accumulates and becomes more localized towards the center of the nucleus, however around the G1/S boundary the staining pattern changes and ORC1 appears peripheral. By mid to late S phase ORC1 immunofluorescence is again concentrated at the nuclear center. During anaphase, ORC1 staining is localized mainly in the pericentriolar regions. These findings suggest that concerted movements of origin DNA sequences in addition to the previously documented assembly and disassembly of protein complexes are an important aspect of replication initiation loci in eukaryotes.
机译：起源识别复合体（ORC）与复制起源的相互作用是真核复制起始过程中的关键参数。在哺乳动物中，除了有丝分裂期间，ORC仍然保持结合状态，因此ORC复合体的定位可以定位来源。识别人类ORC1的单克隆抗体被用于定位ORC复合体在人类MOLT-4细胞群体中的位置，该群体通过离心淘析被细胞周期位置分隔开。 G1早期细胞中的ORC1染色是弥漫性的，主要是外周性的。当细胞穿过G1时，ORC1聚集并变得更靠近核中心，但是在G1 / S边界附近，染色模式发生了变化，ORC1出现在外围。到S期中后期，ORC1免疫荧光再次集中在核中心。在后期，ORC1染色主要定位在中央小周区。这些发现表明，除了先前记录的蛋白质复合物的组装和拆卸之外，起源DNA序列的一致运动是真核生物中复制起始基因座的重要方面。

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