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首页> 外文期刊>Cellular Oncology: Analytical Cellular Pathology >Proteasome (Prosome) Subunit Variations during the Differentiation of Myeloid U937 Cells
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Proteasome (Prosome) Subunit Variations during the Differentiation of Myeloid U937 Cells

机译：骨髓U937细胞分化过程中的蛋白酶体（Prosome）亚基变异。

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20S proteasomes (prosomes/multicatalytic proteinase) are protein particles built of 28 subunits in variable composition. We studied the changes in proteasome subunit composition during the differentiation of U937 cells induced by phorbol‐myristate‐acetate or retinoic acid plus 1,25‐dihydroxy‐cholecalciferol by western blot, flow cytometry and immuno‐fluorescence. p25K (C3), p27K (IOTA) and p30/33K (C2) subunits were detected in both the nucleus and cytoplasm of undifferentiated cells. Flow cytometry demonstrated a biphasic decrease in proteasome subunits detection during differentiation induced by RA+VD. PMA caused an early transient decrease in these subunits followed by a return to their control level, except for p30/33K, which remained low. Immuno‐fluorescence also showed differences in the cytolocalization of the subunits, with a particular decrease in antigen labeling in the nucleus of RA+VD‐induced cells, and a scattering in the cytoplasm and a reorganization in the nucleus of PMA‐induced cells. Small amounts of proteasomal proteins were seen on the outer membrane of non‐induced cells; these membrane proteins disappeared when treated with RA+VD, whereas some increased on PMA‐induced cells. The differential changes in the distribution and type of proteasomes in RA+VD and PMA‐induced cells indicate that, possibly, 20S proteasomes may play a role in relation to the mechanisms of differentiation and the inducer used.
机译：20S蛋白酶体（prosomes /多催化蛋白酶）是由28个亚基组成可变的蛋白质颗粒。我们通过蛋白质印迹，流式细胞术和免疫荧光研究了佛波-肉豆蔻酸酯-乙酸或视黄酸加1,25-二羟基胆钙化固醇诱导的U937细胞分化过程中蛋白酶体亚基组成的变化。在未分化细胞的细胞核和细胞质中均检测到p25K（C3），p27K（IOTA）和p30 / 33K（C2）亚基。流式细胞仪显示在由RA + VD诱导的分化过程中，蛋白酶体亚基的检测双相减少。 PMA导致这些亚基的早期短暂减少，随后恢复至其控制水平，但p30 / 33K仍然很低。免疫荧光还显示了亚基的细胞定位差异，其中RA + VD诱导的细胞核中抗原标记的特别减少，细胞质中的分散和PMA诱导的细胞核中的重组。在非诱导细胞的外膜上可见少量的蛋白酶体蛋白。当用RA + VD处理时，这些膜蛋白消失了，而在PMA诱导的细胞上则增加了。 RA + VD和PMA诱导的细胞中蛋白酶体分布和类型的差异变化表明，可能20S蛋白酶体可能与分化机制和所用诱导剂有关。

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