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首页> 外文期刊>Cellular Oncology: Analytical Cellular Pathology >Molecular Detection of Minimal Residual Cancer in Surgical Margins of Head and Neck Cancer Patients
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Molecular Detection of Minimal Residual Cancer in Surgical Margins of Head and Neck Cancer Patients

机译：头颈部癌症患者手术切缘中最小残留癌的分子检测

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A great disappointment in head and neck cancer surgery is that 10–30% of head and neck squamous cell carcinoma (HNSCC) patients develop local recurrences despite histopathologically tumor-free surgical margins. These recurrences result from either minimal residual cancer (MRC) or preneoplastic lesions that remain behind after tumor resection. Distinguishing MRC from preneoplasic lesions is important to tailor postoperative radiotherapy more adequately. Here we investigated the suitability of quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) using human Ly-6D (hLy-6D) transcripts as molecular marker to detect MRC in surgical margins.Submucosal samples of deep surgical margins were collected from 18 non-cancer control patients and 67 HNSCC patients of whom eight had tumor-positive surgical margins. The samples were analyzed with hLy-6D qRT-PCR, and the data were analyzed in relation to the clinicohistological parameters.A significant difference was shown between the group of patients with histopathological tumor-positive surgical margins and the non-cancer control group (p< 0.001), and the group of patients with histopathological tumor-free surgical margins (p= 0.001).This study shows a novel approach for molecular analysis of deep surgical margins in head and neck cancer surgery. The preliminary data of this approach for detection of MRC in deep margins of HNSCC patients are promising.
机译：头颈癌手术的一大失望之处在于，尽管有组织病理学上无肿瘤的手术切缘，但仍有10-30％的头颈鳞状细胞癌（HNSCC）患者出现局部复发。这些复发是由于肿瘤切除后残留的微小残留癌瘤（MRC）或肿瘤前病变所致。区分MRC和新肿瘤前病变对于更充分地调整术后放疗很重要。在这里我们调查了以人类Ly-6D（hLy-6D）转录本为分子标记的定量逆转录聚合酶链反应（qRT-PCR）的适用性，以检测手术切缘中的MRC。从18个非手术切缘中收集了深层手术切缘的粘膜下样品-癌症对照患者和67例HNSCC患者，其中8例具有肿瘤阳性手术切缘。使用hLy-6D qRT-PCR分析样品，并分析与临床组织学参数相关的数据。组织病理学肿瘤阳性手术切缘患者组与非癌对照组（p <0.001），以及具有组织病理学无肿瘤手术切缘的患者组（p = 0.001）。本研究显示了一种用于头颈癌手术深层手术切缘的分子分析的新方法。该方法用于HNSCC患者深缘MRC检测的初步数据很有希望。

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