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首页> 外文期刊>Cellular Physiology and Biochemistry >SUMO-Specific Protease 2 Suppresses Cell Migration and Invasion through Inhibiting the Expression of MMP13 in Bladder Cancer Cells
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SUMO-Specific Protease 2 Suppresses Cell Migration and Invasion through Inhibiting the Expression of MMP13 in Bladder Cancer Cells

机译：SUMO特异蛋白酶2通过抑制膀胱癌细胞MMP13的表达抑制细胞迁移和侵袭

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Background: SUMO-specific protease 2 (SENP2) is a de-SUMOylation protease family member which has an indispensable role in the regulation of NF-κB transcriptional activation and Wnt signaling. However, whether SENP2 plays a role in tumor metastasis is completely unknown. Methods: Real-time PCR and Western blot was used to detect the expression of SENP2 in human bladder cancer samples and cell lines. Small interfering RNA (siRNA) was used to silencing the expression of SENP2. Matrigel-coated invasion chambers were used to detect the invasion ability of SENP2 in bladder cancer cells. Results: SENP2 was down-regulated in bladder cancer samples. SENP2 inhibited bladder cancer cells migration and invasion in vitro. Transcriptional analysis of several genes associated with tumor metastasis and invasion demonstrated that SENP2 selectively down-regulated MMP13 in bladder cancer cells. Further analysis indicated that silencing of MMP13 rescued the invasive phenotype in SENP2 expressing T24 cells. Conclusion: SENP2 functions as a tumor metastasis suppressor in bladder cancer. The effects of SENP2 on bladder cancer invasion are partially mediated by inhibiting the expression of MMP13.
机译：背景：SUMO特异性蛋白酶2（SENP2）是SUMOylation蛋白酶家族成员，在调节NF-κB转录激活和Wnt信号传导中起着不可或缺的作用。但是，SENP2是否在肿瘤转移中起作用尚不清楚。方法：采用实时荧光定量PCR和Western blot检测SENP2在膀胱癌样品和细胞株中的表达。小干扰RNA（siRNA）用于沉默SENP2的表达。涂有基质胶的侵袭室用于检测SENP2在膀胱癌细胞中的侵袭能力。结果：SENP2在膀胱癌样品中被下调。 SENP2在体外抑制膀胱癌细胞的迁移和侵袭。对与肿瘤转移和侵袭相关的几个基因的转录分析表明，SENP2在膀胱癌细胞中选择性下调了MMP13。进一步的分析表明，沉默MMP13可以拯救SENP2表达T24细胞的侵袭性表型。结论：SENP2可作为膀胱癌的肿瘤转移抑制因子。 SENP2对膀胱癌侵袭的影响部分由抑制MMP13的表达介导。

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