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首页> 外文期刊>Cellular Physiology and Biochemistry >MicroRNA-141 Targets Sirt1 and Inhibits Autophagy to Reduce HBV Replication
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MicroRNA-141 Targets Sirt1 and Inhibits Autophagy to Reduce HBV Replication

机译：MicroRNA-141靶向Sirt1并抑制自噬以减少HBV复制

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>Background/Aims: About 400 million individuals are chronically infected with hepatitis B virus, at high risk of developing liver cirrhosis and hepatocellular carcinoma. Recent studies have demonstrated an interaction between hepatitis B virus replication and autophagy activity of hepatocytes. In the present study, we aimed to investigate the role of miR-141 in regulating autophagy and hepatitis B virus replication. Methods: The expression of HBV-DNA, miR-141 and Sirt1 mRNA was determined by quantitative real-time PCR analysis. The expression of HBsAg and HBeAg was determined by ELISA. Western blotting was performed to detect protein expression. The LC3 puncta was determined by immunofluorescence. To test whether miR-141 directly regulate the expression level of Sirt1 mRNA, dual-luciferase reporter gene assay was performed. Results: In vitro studies showed that miR-141 mimic inhibited the autophagic response, hepatitis B virus and the expression of Sirt1 in hepatocytes. And transfection with miR-141 inhibitor enhanced autophagic response and Sirt1 expression. The autophagy induced by overexpression of Sirt1 was inhibited by miR-141 mimic. In addition, miR-141 mimic also decreased the expression of Sirt1 mRNA. Sirt1 was predicted as a potential miR-141 target by bioinformatic analysis of its 3'-UTR, and confirmed by luciferase reporter assays which analyzing the interaction of miR-141 with the wild- type or the mutated Sirt1 3a€?-UTR. Conclusion: We have therefore demonstrated a role of miR-141 in regulating autophagy-mediated hepatitis B virus inhibition by targeting Sirt1, and may provide potential targets for drug development.
机译：> 背景/目标 ：大约有4亿个体长期感染高风险的乙型肝炎病毒发展为肝硬化和肝细胞癌。最近的研究表明乙型肝炎病毒复制与肝细胞自噬活性之间存在相互作用。在本研究中，我们旨在研究miR-141在调节自噬和乙型肝炎病毒复制中的作用。 方法 ： 实时定量检测HBV-DNA，miR-141和Sirt1 mRNA的表达PCR分析。 ELISA法检测HBsAg和HBeAg的表达。进行蛋白质印迹以检测蛋白质表达。通过免疫荧光测定LC3点。为了测试miR-141是否直接调节Sirt1 mRNA的表达水平，进行了双重荧光素酶报告基因检测。 结果 ： 体外研究表明，miR-141模拟物可抑制自噬反应，乙型肝炎病毒和Sirt1在肝细胞中的表达。用miR-141抑制剂转染可增强自噬反应和Sirt1表达。 SirR1的过表达诱导的自噬被miR-141模拟物抑制。另外，miR-141模拟物也降低了Sirt1 mRNA的表达。通过对其3'-UTR的生物信息学分析，将Sirt1预测为潜在的miR-141靶标，并通过萤光素酶报告基因分析证实了Sirt1，其分析了miR-141与野生型或突变的Sirt13aβ-UTR的相互作用。 结论 ： 因此，我们证明了miR-141在调节自噬介导的乙型肝炎病毒抑制作用中的作用以Sirt1为目标，并可能为药物开发提供潜在的目标。

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