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首页> 外文期刊>Cellular Physiology and Biochemistry >Phospholipid Scramblase 1 Functionally Interacts with Angiogenin and Regulates Angiogenin-Enhanced rRNA Transcription
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Phospholipid Scramblase 1 Functionally Interacts with Angiogenin and Regulates Angiogenin-Enhanced rRNA Transcription

机译：磷脂Scramblase 1在功能上与血管生成素相互作用并调节血管生成素增强的rRNA转录。

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biBackground /i/bAngiogenin (ANG) can translocate to the target cell nucleus and accumulate in the nucleolus to enhance rRNA transcription, thus promoting cell proliferation. However, the regulation of ANG-enhanced rRNA transcription remains unknown. Previously we identified phospholipid scramblase 1 (PLSCR1) as a potential ANG-interacting protein in yeast two-hybrid screening. biMethods /i/bThe interaction was re-confirmed in yeast cells and further verified by iin vitro/i pull down, iin vivo/i co-immunoprecipitation (Co-IP), fluorescent resonance energy transfer (FRET) and immunofluorescence analyses. The rRNA transcription level was determined by real-time quantitative PCR and Northern blot. biResults /i/bPLSCR1 was identified as a novel ANG-interacting protein. Notably, PLSCR1 interacted with ANG in the cell nucleus and regulated rRNA transcription. Furthermore, depletion of cellular ANG expression abolished PLSCR1-enhanced rRNA transcription, which could be rescued by exogenous ANG. biConclusion /i/bOur data suggest that PLSCR1 positively regulates rRNA transcription through interacting with ANG, thus deepening our understanding on rRNA transcription regulation.
机译：背景 血管生成素（ANG）可以转运到靶细胞核并在核仁中积累以增强rRNA转录，从而促进细胞增殖。然而，ANG增强rRNA转录的调控仍然是未知的。以前，我们在酵母双杂交筛选中鉴定出磷脂加扰酶1（PLSCR1）为潜在的ANG相互作用蛋白。 方法 在酵母细胞中再次确认了相互作用，并通过体外下拉，体内 -免疫沉淀（Co-IP），荧光共振能量转移（FRET）和免疫荧光分析。通过实时定量PCR和Northern印迹确定rRNA转录水平。 结果 PLSCR1被鉴定为新型ANG相互作用蛋白。值得注意的是，PLSCR1与细胞核中的ANG相互作用并调节rRNA转录。此外，细胞ANG表达的耗竭消除了PLSCR1增强的rRNA转录，这可以通过外源ANG挽救。 结论 我们的数据表明PLSCR1通过与ANG相互作用来积极调节rRNA转录，从而加深了我们对rRNA转录调控的理解。

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