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首页> 外文期刊>Cellular Physiology and Biochemistry >External Qi of Yan Xin Qigong Inhibits Activation of Akt, Erk1/2 and NF-?B and Induces Cell Cycle Arrest and Apoptosis in Colorectal Cancer Cells
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External Qi of Yan Xin Qigong Inhibits Activation of Akt, Erk1/2 and NF-?B and Induces Cell Cycle Arrest and Apoptosis in Colorectal Cancer Cells

机译：欣欣气功的外气抑制大肠癌细胞Akt，Erk1 / 2和NF-κB的活化并诱导细胞周期阻滞和凋亡

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Background/Aims: Colorectal cancer (CRC) is the second leading cause of cancer death in the Western countries. Novel approaches of treatment are needed for CRC. The purpose of the present study was to investigate cytotoxic effect of external Qi of Yan Xin Qigong (YXQ-EQ) on human colorectal cancer cells. Methods: The effect of YXQ-EQ on viability, cell cycle progression and apoptosis in colorectal cancer HT-29 cells was investigated. Phosphorylation of Akt and Erk1/2, activation of NF-ĸB and the expression of proteins involved in regulation of cell cycle and apoptosis were examined by Western blot analysis. Results: YXQ-EQ markedly decreased viability and blocked colony formation of HT-29 cells. YXQ-EQ downregulated cyclin D1 expression and increased accumulation of cyclin-dependent kinase inhibitors p21Cip1 and p27Kip1, resulting in G1 cell cycle arrest. YXQ-EQ induced apoptosis in HT-29 cells in association with decreased expression of antiapoptotic proteins Bcl-xL, XIAP, survivin and Mcl-1 and elevated expression of proapoptotic protein Bax. YXQ-EQ significantly repressed phosphorylation of Akt and Erk1/2 and NF-ĸB activation in HT-29 cells, suggesting that YXQ-EQ may exert cytotoxic effect through regulating signaling pathways critical for cell proliferation and survival. Furthermore, YXQ-EQ treated PBS and an YXQ-EQ treated plant extract induced apoptosis in HT-29 cells. Conclusion: These findings show that YXQ-EQ has potent cytotoxic effect on HT-29 cells and suggest that YXQ-EQ could be potentially used for colorectal cancer treatment either directly or indirectly via carriers.
机译：背景/目的：结直肠癌（CRC）是西方国家第二大导致癌症死亡的主要原因。 CRC需要新颖的治疗方法。本研究的目的是研究延心气功（YXQ-EQ）的外气对人大肠癌细胞的细胞毒作用。方法：研究YXQ-EQ对结直肠癌HT-29细胞活力，细胞周期进程和凋亡的影响。通过Western blot分析检测Akt和Erk1 / 2的磷酸化，NF-κB的活化以及调节细胞周期和凋亡的蛋白质的表达。结果：YXQ-EQ显着降低了HT-29细胞的活力并阻止了集落形成。 YXQ-EQ下调细胞周期蛋白D1的表达并增加细胞周期蛋白依赖性激酶抑制剂p21 Cip1 和p27 Kip1 的积累，从而导致G1细胞周期停滞。 YXQ-EQ诱导HT-29细胞凋亡，与抗凋亡蛋白Bcl-xL，XIAP，survivin和Mcl-1的表达降低以及促凋亡蛋白Bax的表达升高有关。 YXQ-EQ显着抑制HT-29细胞中Akt和Erk1 / 2的磷酸化以及NF-ĸB的活化，这表明YXQ-EQ可能通过调节对细胞增殖和存活至关重要的信号通路发挥细胞毒性作用。此外，YXQ-EQ处理的PBS和YXQ-EQ处理的植物提取物诱导HT-29细胞凋亡。结论：这些发现表明，YXQ-EQ对HT-29细胞具有强大的细胞毒性作用，并表明YXQ-EQ可能直接或通过载体间接用于大肠癌治疗。

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