• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cellular Physiology and Biochemistry >MicroRNA Expression Profiling of Pancreatic Cancer Cell Line L3.6p1 Following B7-H4 Knockdown
【24h】

MicroRNA Expression Profiling of Pancreatic Cancer Cell Line L3.6p1 Following B7-H4 Knockdown

机译：B7-H4击倒后胰腺癌细胞系L3.6p1的MicroRNA表达谱

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

>Background/Aims: Co-stimulating molecule B7-H4 regulates T cell-mediated immune responses, participates in tumor immune escape, and promotes the proliferation and metastasis of pancreatic cancer cells. However, the specific mechanisms are unclear. MicroRNAs (miRNAs) participated in the pathogenesis and progression of cancer. Methods: In this study, a microarray technique was used to screen B7-H4-related differentially expressed miRNAs in a pancreatic cancer cell line find those associated with pancreatic cancer. Using a miRCURYTM LNA Array approach, we compared the miRNA expression profiles of L3.6p1 pancreatic cancer cells transfected with B7-H4 siRNA for 72 h with those transfected with non-target siRNAs. Results: B7-H4 siRNA significantly up-regulated 57 miRNAs and down-regulated 14 miRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway analysis of predicted miRNA targets showed that these genes were mainly involved in protein binding, pathways in cancer, mitogen-activated protein kinase (MAPK) signaling pathway, and phosphatidylinositol 3-kinase-Akt (PI3K-Akt) signaling pathway. Conclusions: This is the first description of target genes of B7-H4, showing that miRNAs participate in the B7-H4 mediated regulation of oncogenicity and pathogenesis of pancreatic cancer. These results may help us better understand the role of B7-H4 in the progression of pancreatic cancer and its possible mechanisms. We also provide novel biomarkers for potential treatments of pancreatic cancer.
机译：> 背景/目标： 共同刺激分子B7-H4调节T细胞介导的免疫反应，参与肿瘤免疫逃逸，并促进胰腺的增殖和转移。癌细胞。但是，具体机制尚不清楚。 MicroRNA（miRNA）参与了癌症的发病机理和进展。 方法： 在这项研究中，微阵列技术被用于筛选胰腺癌细胞系中与胰腺癌相关的B7-H4相关差异表达的miRNA。我们使用miRCURY TM LNA Array方法，比较了用B7-H4 siRNA转染72 h的L3.6p1胰腺癌细胞与用非靶siRNA转染的胰腺癌细胞的miRNA表达谱。 结果： B7-H4 siRNA显着上调了57个miRNA，而下调了14个miRNA。基因本体论（GO）和《京都议定书》的基因与基因组百科全书（KEGG）对预测的miRNA靶标进行的途径分析表明，这些基因主要参与蛋白质结合，癌症途径，促分裂原活化蛋白激酶（MAPK）信号传导途径和磷脂酰肌醇3 -激酶-Akt（PI3K-Akt）信号通路。 结论： 这是B7-H4靶基因的第一个描述，表明miRNA参与B7-H4介导的胰腺癌致癌性和发病机制的调控。这些结果可能有助于我们更好地了解B7-H4在胰腺癌进展中的作用及其可能的机制。我们还为胰腺癌的潜在治疗提供了新的生物标记。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号