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Dual Functional Characteristic of Human Aquaporin 10 for Solute Transport

机译：人类水通道蛋白10用于溶质运输的双重功能特性

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Background/Aims Although aquaglyceroporins have been generally believed to operate in a channel mode, which is of nonsaturable nature, for glycerol as well as for water, we recently found that human aquaporin 9 (hAQP9) operates in a carrier-mediated mode, which is of saturable nature, for glycerol. Based on the finding, we assumed that such a characteristic might be shared by the other aquaglyceroporins and examined the functional characteristics of hAQP10, which is an intestine-specific aquaglyceroporin. Methods Transport assays were conducted using iXenopus laevis/i oocytes expressing hAQP10 derived from the microinjected cRNA. Results The transport of glycerol by hAQP10 was found to be highly saturable with a Michaelis constant of 10.4 µM and specifically inhibited by several glycerol analogs such as monoacetin. Furthermore, when glycerol was preloaded in hAQP10-expressing oocytes, its efflux was itrans/i-stimulated by extracellular glycerol. These results indicate the involvement of a carrier-mediated mechanism in glycerol transport by hAQP10. Interestingly, a channel mechanism was also found to be involved in part in hAQP10-mediated glycerol transport. Conclusion The present study unveiled the uniquely dual functional characteristic of hAQP10 as a carrier/channel for solute transport, providing a novel insight into its operation mechanism, which would help further elucidate its physiological role.
机译：背景/目的尽管人们普遍认为水甘油糖蛋白对甘油以及对于水而言都是不饱和的通道模式，但我们最近发现人水通道蛋白9（hAQP9）在载体介导的模式下操作，即甘油的饱和性质。基于这一发现，我们假设其他水甘油糖蛋白可能具有这种特征，并检查了hAQP10的功能特征，hAQP10是一种肠特异性水甘油糖蛋白。方法使用表达自微注射cRNA的hAQP10的非洲爪蟾卵母细胞进行转运测定。结果发现hAQP10对甘油的运输具有高度饱和性，其Michaelis常数为10.4 µM，并被几种甘油类似物（如单乙酸甘油酯）特异性抑制。此外，当甘油预装在表达hAQP10的卵母细胞中时，其外排由细胞外甘油ans>刺激。这些结果表明，hAQP10参与了甘油介导的载体介导的机制。有趣的是，还发现通道机制部分参与hAQP10介导的甘油转运。结论本研究揭示了hAQP10作为溶质运输的载体/通道的独特双重功能特性，提供了对其运作机制的新颖见解，这将有助于进一步阐明其生理作用。

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