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首页> 外文期刊>Cellular Physiology and Biochemistry >Effects of Baicalin on Diabetic Cardiac Autonomic Neuropathy Mediated by the P2Y12 Receptor in Rat Stellate Ganglia
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Effects of Baicalin on Diabetic Cardiac Autonomic Neuropathy Mediated by the P2Y12 Receptor in Rat Stellate Ganglia

机译：黄ical苷对大鼠星状神经节P2Y12受体介导的糖尿病性自主神经病变的影响

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Background/Aims Chronic diabetic hyperglycemia can damage various of organ systems and cause serious complications. Although diabetic cardiac autonomic neuropathy (DCAN) is the primary cause of death in diabetic patients, its pathogenesis remains to be fully elucidated. Baicalin is a flavonoid extracted from Scutellaria baicalensis root and has antibacterial, diuretic, anti-inflammatory, anti- metamorphotic, and antispasmodic effects. Our study explored the effects of baicalin on enhancing sympathoexcitatory response induced by DCAN via the P2Y12 receptor. Methods A type 2 diabetes mellitus rat model was induced by a combination of diet and streptozotocin. Serum epinephrine was measured by enzyme-linked immunosorbent assay. Blood pressure and heart rate were measured using the indirect tail-cuff method. Heart rate variability was analyzed using the frequency-domain of electrocardiogram recordings. The expression levels of P2Y12, interleukin-1beta (IL-1β), tumor necrosis factor alpha (TNF-α), and connexin 43 (Cx43) were determined by quantitative real-time reverse transcription-polymerase chain reaction and western blotting. The interaction between baicalin and P2Y12 determined using by molecular docking. Results Baicalin alleviated elevated blood pressure and heart rate, improved heart rate variability, and decreased the elevated expression levels of P2Y12, IL-1β, TNF-α, and Cx43 in the stellate ganglia of diabetic rats. Baicalin also reduced the elevated concentration of serum epinephrine and the phosphorylation of p38 mitogen-activated protein kinase in diabetic rats. Conclusion Baicalin decreases sympathetic activity by inhibiting the P2Y12 receptor in stellate ganglia satellite glial cells to maintain the balance between sympathetic and parasympathetic nerves and relieves DCAN in the rat.
机译：背景/目的慢性糖尿病性高血糖会损害各种器官系统并引起严重的并发症。尽管糖尿病性心脏自主神经病（DCAN）是糖尿病患者死亡的主要原因，但其发病机理仍有待充分阐明。黄ical苷是从黄S根中提取的黄酮类化合物，具有抗菌，利尿，抗炎，抗变态和解痉作用。我们的研究探索了黄ical苷对增强DCAN通过P2Y12受体引起的交感兴奋反应的作用。方法饮食和链脲佐菌素联合诱导形成2型糖尿病大鼠模型。通过酶联免疫吸附测定法测定血清肾上腺素。使用间接尾袖法测量血压和心率。使用心电图记录的频域分析心率变异性。通过实时定量逆转录-聚合酶链反应和蛋白质印迹法确定P2Y12，白介素-1β（IL-1β），肿瘤坏死因子α（TNF-α）和连接蛋白43（Cx43）的表达水平。黄ical苷和P2Y12之间的相互作用通过分子对接确定。结果黄ical苷可减轻糖尿病大鼠星状神经节中血压和心率的升高，改善心率变异性，并降低P2Y12，IL-1β，TNF-α和Cx43的表达水平。黄ical苷还可以降低糖尿病大鼠的血清肾上腺素浓度升高和p38丝裂原活化蛋白激酶的磷酸化。结论黄ical苷可通过抑制星状神经节卫星神经胶质细胞中的P2Y12受体来降低交感神经活性，从而维持交感神经和副交感神经之间的平衡，并减轻大鼠的DCAN。

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