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首页> 外文期刊>Cell stress & chaperones >Comparison of epitope specificity of anti-heat shock protein 60/65 IgG type antibodies in the sera of healthy subjects, patients with coronary heart disease and inflammatory bowel disease
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Comparison of epitope specificity of anti-heat shock protein 60/65 IgG type antibodies in the sera of healthy subjects, patients with coronary heart disease and inflammatory bowel disease

机译：健康受试者，冠心病和炎性肠病患者血清中抗热休克蛋白60/65 IgG型抗体的表位特异性比较

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Previously, we reported on the presence of antibodies to linear epitopes of human and mycobacterial 60?kD heat shock proteins (HSP) in the sera of healthy blood donors. Since many recent findings indicate that the levels of these antibodies may be altered in coronary heart disease (CHD) and also inflammatory bowel diseases (IBD), it seemed worthwhile to compare the epitope specificity of the anti-HSP60 and anti-HSP65 antibodies in the sera of patients with these diseases to those in healthy subjects. The multipin enzyme-linked immunosorbent assay method was applied with a large overlapping set of synthetic 10-mer peptides covering selected regions of human HSP60 and Mycobacterium bovis HSP65. Sera of 12 healthy persons (HP), 14 CHD, and 14 IBD patients with the same concentration of total anti-HSP60 and HSP65 IgG antibodies were tested. We have identified CHD-specific epitopes in the equatorial domain of the HSP60 protein but in neither region of the HSP65 molecule, indicating that the formation of anti-HSP60 antibodies is not or only partially due to the cross-reaction between human HSP60 and bacterial HSP65. IBD-specific epitopes were found in many regions of the HSP60 and in even more regions of the HSP65 molecule including an IBD-specific T cell epitope in region X as well. These findings indicate that the epitope specificity of the anti-human and anti-mycobacterial HSP60 antibodies associated with various diseases is different.
机译：以前，我们报道了健康献血者血清中存在人类和分枝杆菌60？kD热休克蛋白（HSP）线性表位的抗体。由于许多最新发现表明在冠心病（CHD）和炎症性肠病（IBD）中这些抗体的水平可能会改变，因此似乎有必要比较抗HSP60和抗HSP65抗体在表皮中的表位特异性。这些疾病患者的血清要比健康受试者的血清高。多针酶联免疫吸附测定法适用于覆盖人HSP60和牛分枝杆菌HSP65选定区域的大量合成10-mer肽重叠区。测试了12名健康人（HP），14名CHD和14名IBD患者的血清，这些患者的总抗HSP60和HSP65 IgG抗体浓度相同。我们已在HSP60蛋白质的赤道域中但在HSP65分子的两个区域中均未确定CHD特异性表位，这表明抗HSP60抗体的形成不是或仅部分归因于人HSP60与细菌HSP65之间的交叉反应。在HSP60的许多区域和HSP65分子的更多区域中都发现了IBD特异性表位，在X区域中也发现了IBD特异性T细胞表位。这些发现表明与各种疾病有关的抗人和抗分枝杆菌HSP60抗体的表位特异性是不同的。

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