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首页> 外文期刊>Cellular Physiology and Biochemistry >Comprehensive Analysis of the Chemical Composition and In Vitro Cytotoxic Mechanisms of Pallines Spinosa Flower and Leaf Essential Oils Against Breast Cancer Cells
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Comprehensive Analysis of the Chemical Composition and In Vitro Cytotoxic Mechanisms of Pallines Spinosa Flower and Leaf Essential Oils Against Breast Cancer Cells

机译：花粉刺花叶精油对乳腺癌细胞化学成分及体外细胞毒作用机理的综合分析

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>Background/Aims: In our quest for new natural anticancer agents, we studied the cytotoxicity of the essential oils extracted from flowers and leaves of Pallines spinosa. Methods: The essential oils were extracted by hydrodistillation and solid phase microextraction (SPME) from flowers and leaves of the plant and their composition was determined by GC/GC-MS. The cytotoxicity of the oils was evaluated against MCF-7 and MDA-MB-231 breast adenocarcinomas, and the non-cancerous MCF-10-2A cells, using a flow cytometry-based assay Apoptosis was evaluated by flow cytometry, nuclear staining, caspases activation, and Western blotting techniques, and cell cycle by measuring DNA contents. Results: The hydrodistilled flower oil contained mainly sesquiterpenes (96.39%), while the leaf sample was dominated by oxygenated-sesquiterpenes (51.60%) and sesquiterpene-hydrocarbons (34.06%). In contrast, the SPME oil contained mainly monoterpene-hydrocarbons (44.09%) and sesquiterpene-hydrocarbons (34.15%) in the flower and leaf samples, respectively. The cytotoxicity of the flower oil against MCF-7 (IC50 0.25 ?± 0.03 ?μg/mL) and MDA-MB-231 (IC50 0.21 ?± 0.03 ?μg/mL) was much stronger than the leaf oil (IC50 2.4 ?± 0.5 ?μg/mL and 1.5 ?± 0.1 ?μg/mL, respectively). The toxicity of the flower oil was a??5 to 8-times less in normal MCF-10-2A (IC50 1.3 ?± 0.2 ?μg/mL) and blood mononuclear cells (2.80 ?± 0.45 ?μg/mL) as compared to breast and hematological cancer cells, respectively. Both oils induced a caspase-dependent and -independent apoptosis in MCF-7 and MDA-MB-231 cells, and altered the levels of Bcl-2 and Bax proteins. In addition, the oils arrested cell cycle in both cancer cell lines at G0/G1 phase by modulating the expression of cyclin D1, CDK4 and p21 proteins. Conclusion: The cytotoxicity of P. spinosa oils were mediated by apoptosis and cell cycle arrest, suggesting the potential use of their bioactive compounds as natural anticancer compounds.
机译：> 背景/目标： 在寻求新的天然抗癌剂时，我们研究了从 Pallines spinosa >的花和叶中提取的精油的细胞毒性。 / i>。 方法： 通过加氢蒸馏和固相微萃取（SPME）从香叶的花和叶中提取香精油。植物及其组成通过GC / GC-MS确定。使用基于流式细胞术的分析方法评估了油对MCF-7和MDA-MB-231乳腺癌和非癌性MCF-10-2A细胞的细胞毒性。通过流式细胞术，核染色，胱天蛋白酶评估了细胞凋亡活化，蛋白质印迹技术以及通过测量DNA含量的细胞周期。 结果： 经水蒸馏的花油主要含有倍半萜烯（96.39％），而叶片样品则被氧化处理-倍半萜烯（51.60％）和倍半萜碳氢化合物（34.06％）。相比之下，SPME油在花和叶样品中分别主要包含单萜烃（44.09％）和倍半萜烃（34.15％）。花油对MCF-7（IC 50 0.25？±0.03？μg/ mL）和MDA-MB-231（IC 50 0.21？±0.03？ μg/ mL）比叶油要强得多（分别为IC 50 2.4？±0.5？μg/ mL和1.5？±0.1？μg/ mL）。在正常的MCF-10-2A（IC 50 1.3？±0.2？μg/ mL）和血液单核细胞（2.80±±）下，花油的毒性降低了?? 5至8倍。与乳腺癌和血液癌细胞相比，分别为0.45 µg / mL）。两种油均诱导MCF-7和MDA-MB-231细胞中caspase依赖性和非依赖性凋亡，并改变Bcl-2和Bax蛋白的水平。此外，这些油通过调节细胞周期蛋白D1，CDK4和p21蛋白的表达，在G0 / G1期阻止了这两种癌细胞的细胞周期。 结论： P的细胞毒性。菠菜油是由细胞凋亡和细胞周期阻滞介导的，表明其生物活性化合物有可能用作天然抗癌化合物。

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