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首页> 外文期刊>Cellular Physiology and Biochemistry >Paracrine Interaction between Bone Marrow-derived Stem Cells and Renal Epithelial Cells
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Paracrine Interaction between Bone Marrow-derived Stem Cells and Renal Epithelial Cells

机译：骨髓干细胞与肾上皮细胞之间的旁分泌相互作用

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Background/Aims Renal tubular cells are the main target of ischemic insult associated with acute renal injury. Low oxygen and nutrient supplies result in ATP depletion, leading to cell death and loss of renal function. A possible mechanism by which bone marrow-derived cells support renal tissue regeneration relies on the capacity of mononuclear cells (BMMC), particularly mesenchymal stem cells (MSC), to secrete paracrine factors that mediate support for kidney regeneration. Methods BMMC/MSC and renal cells (LLC-PKsub1/sub from pig and IRPTC from rat) were co-cultured under stressful conditions (ATP depletion and/or serum free starvation), physically separated by a microporous membrane (0.4 µm), was used to determine whether bone marrow-derived cells can interact with renal cells in a paracrine manner. Results This interaction resulted in stimulation of renal cell proliferation and the arrest of cell death. MSC elicit effective responses in renal cells in terms of stimulating proliferation and protection. Such effects are observed in renal cells co-cultured with rat BMMC/MSC, an indication that paracrine mechanisms are not entirely species-specific. Conclusion The paracrine action of BMMC/MSC was influenced by a renal cell stimulus released during stress, indicating that cross-talk with injured cells is required for renal regeneration supported by bone marrow-derived cells.
机译：背景/目的肾小管细胞是与急性肾损伤相关的缺血性损伤的主要靶标。氧气和营养物质供应不足会导致ATP耗竭，从而导致细胞死亡和肾功能丧失。骨髓细胞支持肾脏组织再生的可能机制取决于单核细胞（BMMC），尤其是间充质干细胞（MSC）分泌旁分泌因子来介导肾脏再生的能力。方法将BMMC / MSC与肾细胞（猪的LLC-PK 1 和大鼠的IRPTC）在应激条件下（ATP耗竭和/或无血清饥饿）共培养，并通过微孔膜进行物理分离（0.4 µm）用于确定骨髓来源的细胞是否可以旁分泌方式与肾细胞相互作用。结果这种相互作用导致刺激肾细胞增殖并阻止细胞死亡。 MSC在刺激增殖和保护方面引起肾细胞有效反应。在与大鼠BMMC / MSC共培养的肾细胞中观察到了这种作用，这表明旁分泌机制并不完全是物种特异性的。结论BMMC / MSC的旁分泌作用受到应激过程中释放的肾细胞刺激的影响，表明骨髓来源的细胞支持的肾再生需要与受损细胞的串扰。

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