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首页> 外文期刊>Cellular Physiology and Biochemistry >Effect of the Total Extract of Averrhoacarambola (Oxalidaceae) Root on the Expression Levels of TLR4 and NF-κB in Streptozotocin-Induced Diabetic Mice
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Effect of the Total Extract of Averrhoacarambola (Oxalidaceae) Root on the Expression Levels of TLR4 and NF-κB in Streptozotocin-Induced Diabetic Mice

机译：杨桃根总提取物对链脲佐菌素诱导的糖尿病小鼠TLR4和NF-κB表达水平的影响

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Background: Averrhoacarambola L., which is a folk medicine used in diabetes mellitus (DM) in ancient China, has been reported to have anti-diabetic efficacy. Aims: The aim of this study was to evaluate the hypoglycemic effect of the extract of Averrhoacarambola L. root (EACR) on the regulation of the Toll-like receptor 4 (TLR4)-Nuclear-factor kappa B (NF-κB) pathway in B) pathway in streptozotocin (STZ)-induced diabetic mice. Methods: the mice were injected with STZ (120 mg/kg body weight) via a tail vein. After 72 h, the mice with FBG ≥ 11.1 mmol/L were confirmed as having diabetes. Subsequently, the mice were treated intragastrically with EACR (300, 600, 1200 mg/kg body weight/d) and metformin (320 mg/kg body weight/d) for 14 days. Results: As a result the serum fasting blood glucose (FBG), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels were decreased following EACR administration. Immunohistochemical analysis revealed that the pancreatic tissue expression levels of TLR4 and NF-κB were downregulated after EACR administration. EACR suppressed pancreatic mRNA expression level of TLR4 and blocked the downstream NF-κB pathway in the pancreas. According to Western blot analysis EACR suppressed pancreatic TLR4 and NF-κB protein expression levels. Histopathological examination of the pancreas showed that STZ-induced pancreas lesions were alleviated by the EACR treatment. Conclusion: These findings suggest that the modulation of the IL-6 and TNF-α inflammatory cytokines and the suppression of the TLR4-NF-κB pathway are most likely involved in the anti-hyperglycemic effect of EACR in STZ-induced diabetic mice.
机译：背景：据报道，在中国古代用于糖尿病（DM）的民间药物Averrhoacarambola L.具有抗糖尿病功效。目的：本研究旨在评估大叶杨提取物（EACR）对Toll样受体4（TLR4）-核因子κB（NF-κB）途径的调节的降血糖作用。 B）链脲佐菌素（STZ）诱导的糖尿病小鼠中的途径。方法：通过尾静脉给小鼠注射STZ（120 mg / kg体重）。 72小时后，FBG≥11.1 mmol / L的小鼠被确认患有糖尿病。随后，用EACR（300、600、1200 mg / kg体重/ d）和二甲双胍（320 mg / kg体重/ d）胃内治疗小鼠14天。结果：结果，EACR给药后血清空腹血糖（FBG），白介素6（IL-6）和肿瘤坏死因子-α（TNF-α）水平降低。免疫组织化学分析显示，EACR给药后，胰腺组织中TLR4和NF-κB的表达水平下调。 EACR抑制胰腺TLR4的mRNA表达水平，并阻断胰腺下游的NF-κB通路。根据蛋白质印迹分析，EACR抑制胰腺TLR4和NF-κB蛋白表达水平。胰腺的组织病理学检查显示，EACR治疗可减轻STZ诱导的胰腺病变。结论：这些发现表明，STZ诱导的糖尿病小鼠中，IL-6和TNF-α炎性细胞因子的调节以及TLR4-NF-κB途径的抑制很可能与EACR的降血糖作用有关。

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