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首页> 外文期刊>Cellular Physiology and Biochemistry >Osteopontin, Bone Morphogenetic Protein-4, and Vitamin D Receptor Gene Polymorphisms in the Susceptibility and Clinical Severity of Spinal Tuberculosis
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Osteopontin, Bone Morphogenetic Protein-4, and Vitamin D Receptor Gene Polymorphisms in the Susceptibility and Clinical Severity of Spinal Tuberculosis

机译：骨桥蛋白，骨形态发生蛋白4和维生素D受体基因多态性与脊髓结核的易感性和临床严重性

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>Background/Aims: Spinal tuberculosis (TB) is a common and dangerous form of extrapulmonary TB with unclear mechanisms in its occurrence and progression. This study investigated the clinical significances of bone morphogenetic protein-4 (BMP-4), osteopontin (OPN), and vitamin D receptor (VDR) gene polymorphism, mRNA and protein expression in spinal TB patients. Methods: BMP-4 and OPN gene polymorphisms were detected by direct DNA sequencing, while VDR-FokI polymorphisms were analyzed using PCR-RFLP. mRNA and protein expression was measured using real-time PCR and Western blot, respectively. Results: A significant lower frequency of TT genotype and T allele at 6007C>T polymorphism in BMP-4 gene; higher frequency of GG genotype and G allele at -66T>G polymorphism in OPN gene, and higher frequency of the ff genotype and f allele at the VDR-FokI polymorphism were observed in patients with spinal TB compared to controls. TT genotype of 6007C>T polymorphism correlated with a lower BMP-4 mRNA and protein expression, -66GG genotype correlated with a high OPN mRNA and protein expression, and ff genotype correlated with the lower VDR mRNA and protein levels in the intervertebral disc tissues. The TT genotype and low BMP-4 gene expression; the -66GG genotype and high OPN gene expression; and the ff genotype and low VDR gene expression significantly correlated with the clinical severity of spinal TB. Conclusion: The 6007C>T polymorphism of BMP-4, -66T>G polymorphism of OPN, and VDR-FokI polymorphism are the susceptible factors of spinal TB and indicators of the clinical severity. These three genes may collaborate in the development of spinal TB.
机译：> 背景/目标： 脊柱结核（TB）是肺外结核的常见和危险形式，其发生和发展机制尚不清楚。这项研究探讨了骨形态发生蛋白4（ BMP-4 ），骨桥蛋白（ OPN ）和维生素D受体（ VDR ）结核病患者的基因多态性，mRNA和蛋白质表达。 方法： BMP-4 和 OPN 基因多态性通过直接DNA测序检测，而 VDR-使用PCR-RFLP分析FokI多态性。分别使用实时PCR和蛋白质印迹法测量mRNA和蛋白质表达。 结果： BMP-4基因中6007C> T多态性的TT基因型和T等位基因的频率显着降低;与对照组相比，OPN基因中-66T> G多态性的GG基因型和G等位基因频率更高，而VDR-FokI多态性的ff基因型和f等位基因频率更高。 6007C> T多态性的TT基因型与较低的 BMP-4 mRNA和蛋白表达相关，-66GG基因型与较高的 OPN mRNA和蛋白表达相关，与ff基因型相关椎间盘组织中 VDR 的mRNA和蛋白质水平较低。 TT基因型和 BMP-4 基因低表达; -66GG基因型和高 OPN 基因表达; ff基因型和 VDR 基因低表达与脊柱结核的临床严重程度密切相关。 结论： BMP-4的6007C> T多态性，OPN的-66T> G多态性和VDR-FokI多态性是脊髓结核的易感因素，是脊髓结核的指标。临床严重程度。这三个基因可能在脊髓结核的发展中协同作用。

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