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首页> 外文期刊>CHROMATOGRAPHY >Influences of Analyte Injection Volumes and Concentrations on Capillary Chromatography Based on Tube Radial Distribution of Carrier Solvents under Laminar Flow Conditions
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Influences of Analyte Injection Volumes and Concentrations on Capillary Chromatography Based on Tube Radial Distribution of Carrier Solvents under Laminar Flow Conditions

机译：基于层流条件下载体溶剂的管径分布的分析物进样量和浓度对毛细管色谱的影响

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A capillary chromatography called the tube radial distribution chromatography (TRDC) system has been developed using an open capillary tube and a water-hydrophilic/hydrophobic organic solvent mixture carrier solution. In this study, we examined the effects of the analyte injection volume (injection time) and concentration on the chromatograms in the TRDC system using a fused-silica capillary tube (50 μm i.d. and 100 cm effective length) and a water-acetonitrile-ethyl acetate mixture carrier solution (volume ratio, 3: 8: 4). Analyte solutions of 1-naphthol and 2,6-naphthalenedisulfonic acid (1 mM each) were injected into the system with various injection times of 10-1500 s from a height of 20 cm by gravity. They were separated and detected in this order with good reproducibility up to an injection time of 150 s. The analyte solutions (0.075-3.0 mM) were analyzed with the definite injection time of 30 s from a height of 20 cm by gravity. They were separated and detected with a baseline separation and their calibration curves showed linearity up to 1.5 mM. It was confirmed that the TRDC system worked well as a quantitative analysis.
机译：已经开发了一种毛细管色谱法，称为管径向分布色谱法（TRDC），它使用开放式毛细管和水-亲水/疏水性有机溶剂混合物载体溶液制成。在这项研究中，我们使用熔融硅胶毛细管（内径为50μm，有效长度为100 cm）和水-乙腈-乙基溶液检查了分析物进样量（进样时间）和浓度对TRDC系统中色谱图的影响。醋酸盐混合物载体溶液（体积比为3：8：4）。将1-萘酚和2,6-萘二磺酸（各1 mM）的分析物溶液在重力作用下从20 cm的高度注入10-1500 s的各种注入时间。以高达150 s的进样时间以良好的重现性对该样品进行分离和检测。在重力作用下从20 cm的高度以30 s的确定进样时间分析分析物溶液（0.075-3.0 mM）。将它们分离并通过基线分离进行检测，其校准曲线显示线性高达1.5 mM。可以肯定的是，TRDC系统可以很好地进行定量分析。

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