Restriction endonuclease analysis of porcine Pasteurella multocida isolates from Quebec.

J Harel; S C?té; M Jacques

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Restriction endonuclease analysis of porcine Pasteurella multocida isolates from Quebec.

机译：魁北克猪多杀巴斯德氏菌分离株的限制性核酸内切酶分析。

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We have used restriction endonuclease analysis (REA) of genomic DNA to classify porcine Pasteurella multocida isolates with similar capsular and somatic serotypes, and to monitor the distribution of isolates from 12 different herds in Quebec. Within herds, P. multocida isolates of similar capsular and somatic serotypes showed similar REA fingerprints. Between herds, some isolates had similar REA fingerprints. However, differences in REA enabled subtyping of many P. multocida isolates with the same antigen types. Our data indicate that REA would enable accurate epidemiological typing of P. multocida in conjunction with classical capsular and somatic typing.

机译：我们已使用基因组DNA的限制性核酸内切酶分析（REA）对具有相似荚膜和体型血清型的猪多杀巴斯德氏菌分离株进行分类，并监测魁北克12个不同种群的分离株分布。在畜群中，具有相似荚膜和体型血清型的多杀疟原虫分离株显示出相似的REA指纹。在畜群之间，一些分离株具有相似的REA指纹。但是，REA的差异使得许多具有相同抗原类型的多杀毕赤酵母分离株能够亚型化。我们的数据表明，REA将能够结合经典的荚膜和体细胞分型对准确的流行病学分型。

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《Canadian Journal of Veterinary Research》 |1990年第4期|共页
作者
J Harel; S C?té; M Jacques;
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中图分类动物医学（兽医学）;
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4. A molecular approach to the characterization and intra-species differentiation of porcine Pasteurella multocida strains associated with pneumonia and progressive atrophic rhinitis [C] . Boh Chang Lin, Steve Heller, Terry Marco, Annual Meeting of The American Association of Swine Veterinarians . 2012

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5. Comparison of whole genome sequencing to restriction endonuclease analysis and gel diffusion precipitin test of Pasteurella multocida [D] . LeCount, Karen Jeanne. 2016

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6. Restriction endonuclease analysis of porcine Pasteurella multocida isolates from Quebec. [O] . J Harel, S Côté, M Jacques 1990

机译：魁北克猪多杀巴斯德氏菌分离株的限制性核酸内切酶分析。
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Restriction endonuclease analysis of porcine Pasteurella multocida isolates from Quebec.

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