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首页> 外文期刊>CHROMATOGRAPHY >Determination of Asperulosidic Acid and Deacetylasperulosidic Acid in Rat Plasma After Administration of Morinda citrifolia Juice
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Determination of Asperulosidic Acid and Deacetylasperulosidic Acid in Rat Plasma After Administration of Morinda citrifolia Juice

机译：枸Mo子果汁给药后大鼠血浆中阿魏酸和去乙酰基阿魏酸的含量测定

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Determination of asperulosidic acid (AA) and deacetylasperulosidic acid (DAA) in rat plasma after administration of Morinda citrifolia juice by HPLC-UV detection was described. Deproteinized rat plasma (200 ?L) with MeOH for the HPLC analysis was injected. AA and DAA was separated on ZIC~(?)-HILIC (250×4.6 mm, i.d., 5 μm) with a mixture of CH_(3)CN/MeOH/0.1% formic acid aqueous solution (=70:25:5, v/v/v) at a flow rate of 0.75 mL/min. The absorbance of eluate was monitored at 235 nm. Under this condition, the separation of AA and DAA was achieved within 25 min. The calibration curves using plasma spiked with standards indicated good linearity (r ≥0.996) in the range of 0.8-20 ?g/mL for AA and 4-100 μg/mL for DAA, respectively. The limits of quantitation for AA and DAA at a signal-to-noise ratio of 10 were 0.3 and 1.1 μg/mL, respectively. The validation parameters of the method such as recovery (94.9-101.7 %), precisions (less than 7.1 % for intra-day and less than 9.8 % for inter-day) and accuracy (95.0-101.7 %) were acceptable. The analytes in rat plasma were stable after three freeze-thaw cycles or storage at room temperature for up to 6 h. Furthermore, monitoring of AA and DAA after administration of the Morinda citrifolia juice in which AA and DAA were determined, was successfully demonstrated. This is the first report to determine AA and DAA in rat plasma after administration of Morinda citrifolia juice.
机译：描述了在通过HPLC-UV检测法施用柠檬酸莫林达汁之后测定大鼠血浆中的阿魏酸（AA）和去乙酰基阿魏酸（DAA）。注入含有MeOH的去蛋白大鼠血浆（200 µL），用于HPLC分析。在ZIC〜（？）-HILIC（250×4.6 mm，内径，5μm）上用CH_（3）CN / MeOH / 0.1％甲酸水溶液（= 70：25：5， v / v / v），流速为0.75 mL / min。在235nm下监测洗脱液的吸光度。在此条件下，AA和DAA的分离在25分钟内完成。使用掺有标准品的血浆的校准曲线表明，AA的线性良好（r≥0.996），DAA的线性良好，线性范围为4-100μg/ mL。信噪比为10时，AA和DAA的定量限分别为0.3和1.1μg/ mL。该方法的验证参数，如回收率（94.9-101.7％），精度（日内小于7.1％，日间小于9.8％）和准确性（95.0-101.7％）是可以接受的。经过三个冻融循环或在室温下保存长达6小时后，大鼠血浆中的分析物是稳定的。此外，成功证明了在施用了确定了AA和DAA的西番莲果汁之后对AA和DAA的监测。这是在给予柠檬果蝇汁后测定大鼠血浆中AA和DAA的第一份报告。

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