Preliminary Study of Orthogonal Electrochromatography for Simultaneous Two-Dimensional Separation Using a Monolithic Polymer Layer

Hiroaki ITO; Mika MATSUGI; Shinya KITAGAWA; Hajime OHTANI

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Preliminary Study of Orthogonal Electrochromatography for Simultaneous Two-Dimensional Separation Using a Monolithic Polymer Layer

机译：正交电色谱用于整体聚合物层同时二维分离的初步研究

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Fabrication of a monolithic polymer layer, as the stationary phase, equipped with two electrodes was studied for simultaneous two-dimensional separation, combining high performance liquid chromatography and orthogonal electrophoresis. The monolithic polymer layer was prepared between a pair of glass plates by a two-step UV-initiated photo-polymerization. Prior to preparation of the monolithic polymer layer for separation, high-density monolith bars were prepared along the electrodes to suppress the penetration of bubbles formed at the electrodes into the separation monolith. Using the monolithic polymer layer, 2D separation of colored-dyes was successfully achieved by combining gradient HPLC and orthogonal electrophoresis. The 2D separation of proteins was also demonstrated.

机译：研究了装配有两个电极的整体式聚合物层（作为固定相）用于同时二维分离的过程，结合了高效液相色谱和正交电泳技术。通过两步UV引发的光致聚合在一对玻璃板之间制备整体聚合物层。在制备用于分离的整体式聚合物层之前，沿着电极制备高密度的整体式棒，以抑制在电极处形成的气泡渗透到分离式整体中。使用整体式聚合物层，通过结合梯度HPLC和正交电泳成功地完成了染料的二维分离。还证明了蛋白质的二维分离。

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《CHROMATOGRAPHY》 |2016年第2期|共6页
作者
Hiroaki ITO; Mika MATSUGI; Shinya KITAGAWA; Hajime OHTANI;
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正文语种
中图分类化学;
关键词
ElectrophoresisGradient elutionHPLCMonolithic polymer layerTwo-dimensional separation;

机译：电泳梯度洗脱HPLC单分子聚合物层二维分离;

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专利

1. Open-tubular capillary columns with a porous layer of monolithic polymer for highly efficient and fast separations in electrochromatography [J] . Eeltink S, Svec F, Frechet JMJ Electrophoresis: The Official Journal of the International Electrophoresis Society . 2006,第21期

机译：具有整体聚合物多孔层的开管毛细管柱，可在电色谱中实现高效，快速的分离
2. Separation of Peptides and Oligonucleotides Using a Monolithic Polymer Layer and Pressurized Planar Electrophoresis and Electrochromatography [J] . Scott D. Woodward Iva Urbanova David Nurok and Frantisek Svec Analytical Chemistry . 2010,第9期

机译：使用整体式聚合物层，加压平面电泳和电色谱法分离肽和寡核苷酸
3. Separation of Peptides and Oligonucleotides Using a Monolithic Polymer Layer and Pressurized Planar Electrophoresis and Electrochromatography [J] . Scott D. Woodward, Iva Urbanova, David Nurok, Analytical chemistry . 2010,第9期

机译：使用整体式聚合物层，加压平面电泳和电色谱法分离肽和寡核苷酸
4. Chiral monolithic columns for the enantiomeric separation by capillary electrochromatography [C] . Zilin CHEN Beijing conference and exhibition on instrumental analysis;BCEIA . 2001

机译：手性整体柱，通过毛细管电色谱分离对映体
5. Non-polar and affinity monolithic stationary phases for biopolymer separations by capillary electrochromatography and nano-liquid chromatography . [D] . Okanda, Fred Martin. 2006

机译：通过毛细管电色谱和纳米液相色谱分离生物聚合物的非极性和亲和性整体固定相。
6. Monolithic Superhydrophobic Polymer Layer with Photopatterned Virtual Channel for the Separation of Peptides Using Two-Dimensional Thin Layer Chromatography-Desorption Electrospray Ionization Mass Spectrometry [O] . Yehua Han, Pavel Levkin, Irene Abarientos, -1

机译：单层超疏水聚合物层具有光视图虚拟通道用于使用二维薄层色谱 - 解吸电喷雾电离质谱法分离肽
7. Preliminary Study of Orthogonal Electrochromatography for Simultaneous Two-Dimensional Separation Using a Monolithic Polymer Layer [O] . Hiroaki ITO, Mika MATSUGI, Shinya KITAGAWA, 2016

机译：正交电粒度初步研究使用单片聚合物层同时二维分离

Preliminary Study of Orthogonal Electrochromatography for Simultaneous Two-Dimensional Separation Using a Monolithic Polymer Layer

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