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首页> 外文期刊>CHROMATOGRAPHY >A Rapid and Simple UHPLC-UV Method for Quantitative Determination of Erlotinib and Its Active Metabolite OSI-420 in Human Serum, and Its Application in a Non-Small Cell Lung Cancer Patient
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A Rapid and Simple UHPLC-UV Method for Quantitative Determination of Erlotinib and Its Active Metabolite OSI-420 in Human Serum, and Its Application in a Non-Small Cell Lung Cancer Patient

机译：快速，简单的UHPLC-UV方法定量测定人血清中的厄洛替尼及其活性代谢物OSI-420及其在非小细胞肺癌患者中的应用

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摘要

We developed a rapid and simple UHPLC-UV method for quantitative determination of erlotinib and its metabolite, OSI-420, in order to monitor their serum levels in patients with non-small cell lung cancer (NSCLC). Erlotinib and OSI-420 were extracted from 100 μL of human serum by liquid–liquid extraction using t -butyl methyl ether. The analytes were separated on Inertsil ODS-3 (100 mm × 2.1 mm I.D., 2 μm) as an analytical column using 20 mM potassium phosphate buffer (pH 2.5)/acetonitrile (74:26, v/v) as the mobile phase at a flow rate of 0.6 mL/min, and monitored at a UV wavelength of 345 nm. This method covered a linear concentration range of 6–6000 ng/mL for erlotinib and 6–2000 ng/mL for OSI-420, respectively (r > 0.999). The intra- and inter-day precisions of the analysis were < 6.8%, and the accuracy was ± 7.4%. This method has been successfully applied to measure erlotinib and OSI-420 in the serum of an NSCLC patient.
机译：我们开发了一种快速简便的UHPLC-UV方法，用于定量测定厄洛替尼及其代谢物OSI-420，以监测非小细胞肺癌（NSCLC）患者的血清水平。厄洛替尼和OSI-420是使用叔丁基甲基醚通过液-液萃取法从100μL人血清中提取的。使用20 mM磷酸钾缓冲液（pH 2.5）/乙腈（74:26，v / v）作为流动相，在作为分析柱的Inertsil ODS-3（100 mm×2.1 mm ID，2μm）上分离分析物流速为0.6 mL / min，并在345 nm的UV波长下进行监测。该方法的线性浓度范围为厄洛替尼为6-6000 ng / mL，OSI-420为6-2000 ng / mL（r> 0.999）。分析的日内和日间精度<6.8％，精度为±7.4％。该方法已成功地用于测量NSCLC患者血清中的厄洛替尼和OSI-420。

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