Centriole structure and function in the sea urchin zygote parallel those in mammalian somatic cells. Here, I briefly introduce the properties and attributes of the sea urchin system that make it an attractive platform for the study of centrosome and centriole duplication. These attributes apply to all echinoderms readily available from commercial suppliers: sea urchins, sand dollars, and starfish. I list some of the practical aspects of the system that make it a cost- and time-effective system for experimental work and then list properties that are a “tool kit” that can be used to conduct studies that would not be practical, or in some cases not possible, with mammalian somatic cells. Since centrioles organize and localize the pericentriolar material that nucleates the astral arrays of microtubules (Bobinnec et al. in J Cell Biol 143(6):1575–1589, 1998 ), the pattern of aster duplication over several cell cycles can be used as a reliable measure for centriole duplication (Sluder and Rieder in J Cell Biol 100(3):887–896, 1985 ). Descriptions of the methods my laboratory has used to handle and image echinoderm zygotes are reviewed in Sluder et al. (Methods Cell Biol 61:439–472, 1999 ). Also included is a bibliography of papers that describe additional methods.
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机译：海胆合子中的质心结构和功能与哺乳动物体细胞中的质心结构和功能相似。在这里，我简要介绍了海胆系统的特性和属性，使其成为研究中心体和中心体复制的诱人平台。这些属性适用于可从商业供应商处容易获得的所有棘皮动物：海胆，沙钱和海星。我列出了该系统的一些实用方面，使其成为进行实验工作的具有成本效益和时间效益的系统，然后列出了作为“工具箱”的属性，这些属性可用于进行不实际的研究或在某些情况下，哺乳动物的体细胞是不可能的。由于中心粒组织并定位了微管星形阵列成核的中心粒周围物质（Bobinnec等人，J Cell Biol 143（6）：1575-1589，1998），因此在多个细胞周期中紫复制的模式可以用作中心粒重复的可靠方法（Sluder and Rieder in J Cell Biol 100（3）：887–896，1985）。 Sluder等人综述了我实验室用来处理棘皮动物合子并对其成像的方法的描述。 （Methods Cell Biol 61：439-472，1999）。还包括描述其他方法的论文书目。
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