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Optimization of jenipapo in vitro seed germination process

机译：jenipapo种子萌发过程的优化

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The in vitro seed germination is an effective alternative for quickly obtaining explants with sanitary quality. However, jenipapo seeds present slow and uneven germination. Therefore, internal and external factors to seed which directly interfere in the process, they must be identified, in order to adapt better techniques to obtain seedlings. In this sense, this work aimed to optimize the in vitro germination of Genipa americana L. seeds by evaluating different factors (light quality, GA 3 treatment, pre-soaking in distilled water, growing media and stratification in the dark). It was found that the seed germination of G. americana was indifferent to light, however, the best results were obtained under conditions of continuous darkness; There was no effect of the application of exogenous GA 3 ; The pre-soaking in distilled water for 48 h contributes to obtaining better germination rates; And the reduction in MS medium salts, and laminating the pretreatment in the dark maximizes the germination potential of seeds.Therefore, the optimal conditions for in vitro germination of G. americana L. seeds requires pre-soaking in distilled water for 48 hours and inoculation into culture media consisting of 1/2 MS + 15 g L -1 sucrose, with stratification in the dark for 16 days, followed by the transfer to growth chambers with lighting provided by white fluorescent lamps.
机译：体外种子发芽是快速获得具有卫生品质的外植体的有效替代方法。但是，jenipapo种子发芽缓慢且不均匀。因此，必须确定直接影响种子生长的内部和外部因素，以便采用更好的技术来获得种子。从这个意义上讲，这项工作旨在通过评估不同的因素（光照质量，GA 3处理，预先浸泡在蒸馏水中，生长培养基和在黑暗中分层）来优化美国Genipa L.种子的体外发芽。结果表明，美洲种的种子发芽对光无所谓，但在持续黑暗的条件下可获得最佳结果。外源GA 3的施用没有效果;在蒸馏水中预先浸泡48小时有助于获得更好的发芽率。 MS培养基盐的减少以及在黑暗中进行预处理的层压可最大程度地提高种子的发芽潜力，因此，美国白花蛇舌草种子体外发芽的最佳条件需要在蒸馏水中预先浸泡48小时并接种放入由1/2 MS + 15 g L -1蔗糖组成的培养基中，在黑暗中分层16天，然后转移到白色荧光灯提供的生长室中。

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