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首页> 外文期刊>Circulation journal >Angio-Vasculogenic Properties of Endothelial-Induced Mesenchymal Stem Cells Derived From Human Adipose Tissue
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Angio-Vasculogenic Properties of Endothelial-Induced Mesenchymal Stem Cells Derived From Human Adipose Tissue

机译：内皮诱导的人脂肪组织间充质干细胞的血管血管生成特性

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Background: Although stem cells have been regarded as a promising therapeutic option, the marginal therapeutic effects of stem cells are limitations that must be overcome for the development of effective cell therapy. This study sought to identify the angio-vasculogenic properties of endothelial differentiated mesenchymal stem cells (MSCs) and to determine whether these cells are effective for vascular repair. Methods?and?Results: Adipose MSCs were cultured for 10 days under endothelial cell (EC) culture conditions. These endothelial cell differentiated adipose MSCs (EA) and undifferentiated adipose MSCs (UA) were characterized via angiogenesis and adhesion assays. These cells were transplanted into a hindlimb ischemia (HLI) model to determine therapeutic effects and their underlying mechanisms. EA displayed low adhesion and angiogenic properties in vitro compared with UA. When implanted into mouse HLI models, EA exhibited the decreased recovery of blood perfusion in limb ischemia than uncultured UA. Histology data showed that injected EA exhibited lower retention, angiogenic cytokine levels, and neovascularization in vivo than did UA. Short-term differentiated EA display less cell engraftment and angio-vasculogenic potential, and are less effective for peripheral vascular repair than UA. Conclusions: EC differentiation of MSCs may not present an effective strategy for the promotion of therapeutic neovascularization. ( Circ J 2016; 80: 998–1007)
机译：背景：尽管干细胞被认为是一种有前途的治疗选择，但干细胞的边际治疗效果是发展有效细胞疗法必须克服的局限性。这项研究试图确定内皮分化的间充质干细胞（MSCs）的血管生成特性，并确定这些细胞是否对血管修复有效。方法与结果：将脂肪间充质干细胞在内皮细胞（EC）培养条件下培养10天。这些内皮细胞分化的脂肪间充质干细胞（EA）和未分化的脂肪间充质干细胞（UA）通过血管生成和粘附测定来表征。将这些细胞移植到后肢缺血（HLI）模型中，以确定治疗效果及其潜在机制。与UA相比，EA在体外显示出低的粘附性和血管生成特性。当植入小鼠HLI模型中时，与未培养的UA相比，EA在肢体缺血中的血液灌注恢复降低。组织学数据显示，与UA相比，体内注射的EA在体内表现出更低的保留，血管生成细胞因子水平和新血管形成。短期分化的EA显示出较少的细胞植入和血管生成潜力，并且与UA相比，对外周血管修复的效果较差。结论：MSC的EC分化可能不是促进治疗性新血管形成的有效策略。 （2016年Circ J; 80：998-1007）

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