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首页> 外文期刊>Circulation journal >Combined Analysis of Human and Experimental Murine Samples Identified Novel Circulating MicroRNAs as Biomarkers for Atrial Fibrillation
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Combined Analysis of Human and Experimental Murine Samples Identified Novel Circulating MicroRNAs as Biomarkers for Atrial Fibrillation

机译：人类和实验小鼠样品的组合分析确定了新型循环微RNAs作为房颤的生物标志物

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Background: Recent experimental studies have demonstrated that several microRNAs (miRNAs) expressed in atrial tissue promote a substrate of atrial fibrillation (AF). However, because it has not been fully elucidated whether these experimental data contribute to identifying circulating miRNAs as biomarkers for AF, we used a combined analysis of human serum and murine atrial samples with the aim of identifying these biomarkers for predicting AF. Methods?and?Results: Comprehensive analyses were performed to screen 733 miRNAs in serum from 10 AF patients and 5 controls, and 672 miRNAs in atrial tissue from 6 inducible atrial tachycardia model mice and 3 controls. We selected miRNAs for which expression was detected in both analyses, and their expression levels were changed in the human analyses, the murine analyses, or both. This screening identified 11 candidate miRNAs. Next, we quantified the selected miRNAs using a quantitative RT-PCR in 50 AF and 50 non-AF subjects. The individual assessment revealed that 4 miRNAs (miR-99a-5p, miR-192-5p, miR-214-3p, and miR-342-5p) were significantly upregulated in AF patients. A receiver-operating characteristics curve indicated that miR-214-3p and miR-342-5p had the highest accuracy. The combination of the 4 miRNAs modestly improved the predictive accuracy for AF (76% sensitivity, 80% specificity). Conclusions: Novel circulating miRNAs were upregulated in the serum of AF patients and might be potential biomarkers of AF.
机译：背景：最近的实验研究表明，在心房组织中表达的几种microRNA（miRNA）可以促进房颤（AF）的底物。但是，由于尚未完全阐明这些实验数据是否有助于鉴定循环miRNA作为AF的生物标志物，因此我们对人血清和鼠房样本进行了组合分析，旨在鉴定这些可预测AF的生物标志物。方法和结果：进行了全面分析，筛选了10例AF患者和5例对照的血清中的733 miRNA，以及6例诱导型心动过速模型小鼠和3例对照的心房组织中的672 miRNA。我们选择了在两种分析中均检测到表达的miRNA，并且在人类分析，鼠类分析或两者中都改变了它们的表达水平。该筛选鉴定出11种候选miRNA。接下来，我们使用定量RT-PCR对50名AF和50名非AF受试者进行了定量选择的miRNA。个体评估显示，AF患者中有4种miRNA（miR-99a-5p，miR-192-5p，miR-214-3p和miR-342-5p）显着上调。接收器工作特性曲线表明，miR-214-3p和miR-342-5p具有最高的准确性。 4种miRNA的组合适度提高了AF的预测准确性（灵敏度为76％，特异性为80％）。结论：AF患者血清中新的循环miRNA表达上调，可能是AF的潜在生物标志物。

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