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首页> 外文期刊>Clinical and vaccine immunology: CVI >Inclusion of the Bovine Neutrophil Beta-Defensin 3 with Glycoprotein D of Bovine Herpesvirus 1 in a DNA Vaccine Modulates Immune Responses of Mice and Cattle
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Inclusion of the Bovine Neutrophil Beta-Defensin 3 with Glycoprotein D of Bovine Herpesvirus 1 in a DNA Vaccine Modulates Immune Responses of Mice and Cattle

机译：牛中性粒细胞β-防御素3与DNA疫苗中的牛疱疹病毒1糖蛋白D的结合调节小鼠和牛的免疫反应。

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Bovine herpesvirus 1 (BoHV-1) causes recurrent respiratory and genital infections in cattle and predisposes them to lethal secondary infections. While modified live and killed BoHV-1 vaccines exist, these are not without problems. Development of an effective DNA vaccine for BoHV-1 has the potential to address these issues. As a strategy to enhance DNA vaccine immunity, a plasmid encoding the bovine neutrophil beta-defensin 3 (BNBD3) as a fusion with truncated glycoprotein D (tgD) and a mix of two plasmids encoding BNBD3 and tgD were tested in mice and cattle. In mice, coadministration of BNBD3 on the separate plasmid enhanced the tgD-induced gamma interferon (IFN-γ) response but not the antibody response. BNBD3 fused to tgD did not affect the antibody levels or the number of IFN-γ-secreting cells but increased the induction of tgD-specific cytotoxic T lymphocytes (CTLs). In cattle, the addition of BNBD3 as a fusion construct also modified the immune response. While the IgG and virus-neutralizing antibody levels were not affected, the number of IFN-γ-secreting cells was increased after BoHV-1 challenge, specifically the CD8+ IFN-γ+ T cells, including CD8+ IFN-γ+ CD25+ CTLs. While reduced virus shedding, rectal temperature, and weight loss were observed, the level of protection was comparable to that observed in pMASIA-tgD-vaccinated animals. These data show that coadministration of BNBD3 with a protective antigen as a fusion in a DNA vaccine strengthened the Th1 bias and increased cell-mediated immune responses but did not enhance protection from BoHV-1 infection.
机译：牛疱疹病毒1（BoHV-1）在牛中引起反复的呼吸道和生殖器感染，并使它们易致死性继发感染。尽管存在改良的活活和灭活的BoHV-1疫苗，但它们并非没有问题。为BoHV-1开发有效的DNA疫苗具有解决这些问题的潜力。作为增强DNA疫苗免疫力的策略，在小鼠和牛中测试了编码牛中性粒细胞β-防御素3（BNBD3）与截短糖蛋白D（tgD）融合的质粒以及两种编码BNBD3和tgD的质粒的混合物。在小鼠中，在单独的质粒上共同施用BNBD3可增强tgD诱导的γ干扰素（IFN-γ）反应，但不能增强抗体反应。与tgD融合的BNBD3不会影响抗体水平或分泌IFN-γ的细胞数量，但会增加tgD特异性细胞毒性T淋巴细胞（CTL）的诱导。在牛中，添加BNBD3作为融合构建体也可以改变免疫反应。虽然不影响IgG和病毒中和抗体的水平，但BoHV-1攻击后，分泌IFN-γ的细胞数量增加了，特别是CD8 + IFN-γ + T细胞，包括CD8 + IFN-γ + CD25 + CTL。在观察到病毒脱落，直肠温度降低和体重减轻的同时，保护水平与接种pMASIA-tgD的动物所观察到的水平相当。这些数据表明，将BNBD3与保护性抗原以DNA疫苗的融合形式共同给药可增强Th1偏倚并增加细胞介导的免疫反应，但不会增强对BoHV-1感染的保护。

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