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首页> 外文期刊>Civil and Environmental Engineering Reports >Influence of Cyanobacterial Bloom on Freshwater Biocoenosis. Use of Bioassays for Cyanobacterial Microcystins Toxicity Assessment
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Influence of Cyanobacterial Bloom on Freshwater Biocoenosis. Use of Bioassays for Cyanobacterial Microcystins Toxicity Assessment

机译：蓝藻水华对淡水生物群落的影响。生物测定在蓝藻微囊藻毒素毒性评估中的应用

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The issues presented in this study concern a very important problem of the occurrence of cyanobacterial blooms in surface water used for water supply purposes. The objective of this study was to analyze the occurrence of cyanotoxic risk in the catchment area of the Obrzyca River (including S?awskie lake which is the beginning of the river), which is a source of drinking water for the inhabitants of Zielona Góra. In order to evaluate toxicity of cyanobacterial bloom it was conducted toxicological testing using aquatic invertebrates (Daphnia magna, Dugesia tigrina) and heterotrophic bacteria (Escherichia coli, Enterococcus faecalis, Pseudomonas fluorescens). Test samples were collected from May to October, 2012. The most toxic was a sample collected from Lake S?awskie on 20th October when cyanobacteria bloom with a predominance of Microcystis aeruginosa occurred and the amount of microcystins was the largest. The methanol extract of the sample was toxic only above a concentration of 6·103 mg·dm-3. The lethal concentration (48-h LC 50) for Daphnia magna was 3.09·103 and for Dugesia tigrina (240-h LC 50) 1.51·103 mg·dm-3 of microcystins (MC-LR, MC-YR and MC-RR). The same extract stimulated growth of Escherichia coli and Enterococcus faecalis cells.
机译：这项研究中提出的问题涉及一个非常重要的问题，即用于供水目的的地表水中出现了蓝藻水华。这项研究的目的是分析Obrzyca河（包括作为河流起点的S？awskie湖）集水区的氰毒性风险的发生，该河是ZielonaGóra居民的饮用水来源。为了评估蓝藻水华的毒性，使用了水生无脊椎动物（Daphnia magna，Dugesia tigrina）和异养细菌（Escherichia coli，粪肠球菌，Pseudomonas fluorescens）进行了毒理学测试。从2012年5月至10月收集了测试样品。毒性最高的是10月20日从S？awskie湖采集的样品，当时蓝藻大量繁殖，并以铜绿微囊藻为主，微囊藻毒素的量最大。样品的甲醇提取物仅在6·103 mg·dm-3浓度以上才有毒。大型蚤（Daphnia magna）的致死浓度（48-h LC 50）为3.09·103，虎豚（Dugesia tigrina）（240-h LC 50）的致死浓度为1.51·103 mg·dm-3微囊藻毒素（MC-LR，MC-YR和MC-RR） ）。同一提取物刺激大肠杆菌和粪肠球菌细胞的生长。

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