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Autologous fat grafting: use of closed syringe microcannula system for enhanced autologous structural grafting

机译:自体脂肪移植:使用封闭式注射器微插管系统增强自体结构移植

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Objectives: Provide background for use of acquiring autologous adipose tissue as a tissue graft and source of adult progenitor cells for use in cosmetic plastic surgery. Discuss the background and mechanisms of action of closed syringe vacuum lipoaspiration, with emphasis on accessing adipose-derived mesenchymal/stromal cells and the stromal vascular fraction (SVF) for use in aesthetic, structural reconstruction and regenerative applications. Explain a proven protocol for acquiring high-quality autologous fat grafts (AFG) with use of disposable, microcannula systems. Design: Explain the components and advantage of use of the patented super luer-lock and microcannulas system for use with the closed-syringe system. A sequential explanation of equipment selection for minimally traumatic lipoaspiration in small volumes is presented, including use of blunt injection cannulas to reduce risk of embolism. Results: Thousands of AFG have proven safe and efficacious for lipoaspiration techniques for large and small structural fat grafting procedures. The importance and advantages of gentle harvesting of the adipose tissue complex has become very clear in the past 5 years. The closed-syringe system offers a minimally invasive, gentle system with which to mobilize subdermal fat tissues in a suspension form. Resulting total nuclear counting of undifferentiated cells of the adipose-derived -SVF suggests that the yield achieved is better than use of always-on, constant mechanical pump applied vacuum systems. Conclusion: Use of a closed-syringe lipoaspiration system featuring disposable microcannulas offers a safe and effective means of harvesting small volumes of nonmanipulated adipose tissues and its accompanying progenitor cells within the SVF. Closed syringes and microcannulas are available as safe, sterile, disposable, compact systems for acquiring high-quality AFG. Presented is a detailed, step-by-step, proven protocol for performing quality autologous structural adipose transplantation.
机译:目的:为获取自体脂肪组织作为组织移植物和用于整形外科的成年祖细胞来源提供背景。讨论了封闭式注射器真空吸脂术的背景和作用机理,重点介绍了在美学,结构重建和再生应用中使用脂肪来源的间充质/基质细胞和基质血管部分(SVF)。解释使用一次性微型插管系统获得高质量自体脂肪移植物(AFG)的行之有效的方案。设计:解释与封闭式注射器系统一起使用的专利超级鲁尔锁和微插管系统的组件和优势。介绍了小批量最小创伤性脂肪抽吸术设备选择的顺序说明,包括使用钝针插管以减少栓塞的风险。结果:成千上万的AFG已被证明对于大型和小型结构性脂肪移植程序的脂肪抽吸技术安全有效。在过去5年中,温和收获脂肪组织复合物的重要性和优势已变得十分明显。封闭式注射器系统提供了一种微创,柔和的系统,可用于以悬浮形式动员皮下脂肪组织。对脂肪来源的-SVF的未分化细胞进行的总核计数表明,所获得的产量要好于始终使用恒定机械泵的真空系统。结论:使用具有一次性微插管的封闭注射器脂肪抽吸系统提供了一种安全有效的方法,可在SVF中收集少量未操纵的脂肪组织及其伴随的祖细胞。封闭式注射器和微型插针可作为安全,无菌,一次性,紧凑的系统使用,以获取高质量的AFG。提出的是详细的,逐步的,经过验证的方案,用于执行高质量的自体结构脂肪移植。

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