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Development of a Novel Polymer-Based mRNA Coating for Surgical Suture to Enhance Wound Healing

机译：新型基于聚合物的mRNA涂层的外科手术缝合，以增强伤口愈合的发展。

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A therapeutic strategy to improve wound healing has become an increasingly important medical task due to the rising incidence of adiposity and type II diabetes as well as the proceeding population aging. In order to cope with the resulting burdens, new strategies to achieve rapid and complete wound healing must now be developed. Accordingly, the development of a bioactive wound dressing in the form of a messengerRNA (mRNA)-bearing poly(lactide-co-glycolide acid) (PLGA) coating on surgical suture is being pushed further with this study. Furthermore, the evaluation of the polymer-based transfection reagent Viromer RED has shown that it can be used for the transfection of eukaryotic cells: The mRNA gets properly complexed and translated into a functional protein. In addition, the mRNA-PLGA coating triggered the expression of the keratinocyte growth factor (KGF) in HaCat cells although KGF is not expressed under physiological conditions. Moreover, transfection via surgical sutures coated with mRNA does not affect the cell viability and a proinflammatory reaction in the transfected cells is not induced. These properties make the mRNA-PLGA coating very attractive for the in vivo application. For the future, this could mean that through the use of mRNA-coated sutures in surgical wound closure, cells in the wound area can be transfected directly, thus accelerating and improving wound healing.
机译：由于肥胖症和II型糖尿病的发病率上升以及人口的老龄化，改善伤口愈合的治疗策略已成为越来越重要的医学任务。为了应付由此产生的负担，现在必须开发出实现快速和完全伤口愈合的新策略。因此，这项研究进一步推动了以外科缝合线为载体的具有messengerRNA（mRNA）的聚丙交酯-乙交酯-乙交酯乙交酯酸（PLGA）涂层的生物活性伤口敷料的开发。此外，对基于聚合物的转染试剂Viromer RED的评估表明，它可用于真核细胞的转染：mRNA正确地复合并转化为功能蛋白。另外，尽管在生理条件下不表达KGF，但mRNA-PLGA涂层触发了HaCat细胞中角质形成细胞生长因子（KGF）的表达。而且，通过用mRNA包被的外科缝合线的转染不影响细胞生存力，并且不诱导转染细胞中的促炎反应。这些特性使mRNA-PLGA涂层对于体内应用非常有吸引力。对于未来，这可能意味着通过在手术伤口闭合中使用mRNA涂层缝合线，可以直接转染伤口区域中的细胞，从而加速并改善伤口愈合。

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