...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cogent Food & Agriculture >Detection of foodborne pathogens by qPCR: A practical approach for food industry applications
【24h】

Detection of foodborne pathogens by qPCR: A practical approach for food industry applications

机译：qPCR检测食源性病原体：食品工业应用的实用方法

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Microbiological analysis of food is an integrated part of microbial safety management in the food chain. Monitoring and controlling foodborne pathogens are traditionally carried out by conventional microbiological methods based on culture-dependent approaches in control laboratories and private companies. However, polymerase chain reaction (PCR) has revolutionized microbiological analysis allowing detection of pathogenic microorganisms in food, without the necessity of classical isolation and identification. However, at present, PCR and quantitative polymerase chain reaction (qPCR) are essential analytical tools for researchers working in the field of foodborne pathogens. This manuscript reviews recently described qPCR methods applied for foodborne bacteria detection, serving as economical, safe, and reliable alternatives for application in the food industry and control laboratories. Multiplex qPCR, which allows the simultaneous detection of more than one pathogen in one single reaction, saving considerable effort, time, and money, is emphasized in the article.
机译：食品的微生物学分析是食品链中微生物安全管理的有机组成部分。传统上，在控制实验室和私营公司中，以传统的微生物学方法为基础，通过基于微生物的方法来监测和控制食源性病原体。但是，聚合酶链反应（PCR）彻底改变了微生物分析技术，无需进行经典的分离和鉴定即可检测食品中的病原微生物。但是，目前，PCR和定量聚合酶链反应（qPCR）是在食源性病原体领域工作的研究人员必不可少的分析工具。该手稿对最近描述的qPCR方法用于食源性细菌检测进行了综述，是在食品工业和控制实验室中应用的经济，安全，可靠的替代方法。本文强调了多重qPCR，它允许在一个反应​​中同时检测一种以上的病原体，从而节省了大量的精力，时间和金钱。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号