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首页> 外文期刊>Cogent Food & Agriculture >Allelic variations for lycopene-ε-cyclase and β-carotene hydroxylase genes in maize inbreds and their utilization in β-carotene enrichment programme
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Allelic variations for lycopene-ε-cyclase and β-carotene hydroxylase genes in maize inbreds and their utilization in β-carotene enrichment programme

机译：玉米自交系番茄红素-ε-环化酶和β-胡萝卜素羟化酶基因的等位基因变异及其在β-胡萝卜素富集程序中的利用

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摘要

Vitamin A deficiency is a global health problem and can be effectively alleviated through crop biofortification. Quantification of carotenoids using high-performance liquid chromatography is expensive and time-consuming, thereby posing a challenge in the selection of genotypes with high provitamin A. Favourable alleles possessing rare genetic variation in lycopene -ε -cyclase (lcyE ) and β -carotene hydroxylase (crtRB1 ) genes are associated with higher accumulation of provitamin A, especially β -carotene; and selection of these alleles holds immense promise in reducing large-scale phenotypic assays. Screening of a diverse set of 385 maize inbred lines of indigenous and exotic origin detected the presence of two alleles (amplicon size: 250 and 650?bp) of lcyE and three alleles (amplicon size: 296, 543 and 875?bp) of crtRB1 in the inbred panel. Favourable alleles of both the genes were rare among the traditional maize germplasm; 3.38% of the inbreds possessed the favourable allele (650?bp) of lcyE , while 3.90% inbreds had the favourable allele (543?bp) of crtRB1 . Five inbreds (1.3%) with favourable alleles of both the genes were found. Inbreds with favourable alleles of crtRB1 and lcyE serve as rich genetic resources for effective utilization in the maize biofortification programme.
机译：维生素A缺乏症是一个全球性的健康问题，可以通过作物生物强化来有效缓解。使用高效液相色谱法对类胡萝卜素进行定量分析既昂贵又费时，因此在选择具有高维生素原A的基因型时面临挑战。有利的等位基因在番茄红素-ε-中具有罕见的遗传变异环化酶（lcyE）和β-胡萝卜素羟化酶（crtRB1）基因与维生素原A尤其是β-胡萝卜素的积累有关。这些等位基因的选择在减少大规模表型测定中具有广阔的前景。筛选了385种本地和外来玉米自交系，发现存在两个等位基因（扩增子大小：250和650？bp）的lcyE和三个等位基因（扩增子大小：296、543和875？bp） ）中的 crtRB1）。这两个基因的有利等位基因在传统玉米种质中很少见。 3.38％的自交系具有 lcyE的有利等位基因（650？bp），而3.90％的自交系具有 crtRB1的有利等位基因（543？bp）。发现了具有两个基因的等位基因的五个近交系（1.3％）。具有良好的等位基因crtRB1和lcyE的近交系可作为丰富的遗传资源，在玉米生物强化计划中得到有效利用。

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